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50 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product. ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c 3T3 ...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according to ...
  5. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, ST 20

    The influence of storage conditions and packaging on acetic acid generation in cellulose acetate fibers

    TATUM C.E.; WILSON S.A.; CASPER R.G.; PEARCE M.J.
    Eastman Chemical Company, Kingsport, TN, U.S.A.
    The cellulose acetate polymer used in cigarette filters is made by acetylating wood pulp with acetic anhydride. One advantage of this modified natural polymer is that it readily degrades by multiple mechanisms. While the ultimate products are carbon ...
  6. TSRC, Tob. Sci. Res. Conf., 2015, 69, abstr. 07

    Comparison of the cerulean e-cigarette pressure drop testing instrument to the ces 508 cigarette pressure drop and ventilation tester unit for electronic cigarettes, conventional tobacco burning cigarettes, and filter tips

    AVANTS K.W.(1); WILSON S.A.(2); CLARK J.(1)
    (1) Liggett Group, LLC, Mebane, NC USA; (2) Eastman Chemical Company, Kingsport, TN USA
    The pressure drop or “resistance to draw” of cigarettes and cigarette-like products is a prominent experience for consumers. This poster is a comparison of two commercial instruments with different approaches to measuring pressure drop. The instruments ...
  7. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, PT 01

    Photodegradability testing of cellulose acetate filters – outdoor weathering vs. accelerated weathering

    STEACH J.K.; FISHER JONES D.
    Eastman Chemical Company, Kingsport, TN, USA
    Degradation testing in the laboratory is based on understanding a material’s exposure to specific environmental forces, which can play a key role in the mechanisms of degradation. Cellulose acetate filters can be degraded by various environmental forces, ...