Tobacco Science & Technology, 2010, 7, p. 48-52., ISSN.1002-0861
SRAP analysis of D4 lines of Purslane DNA-introduced tobacco
1. College of Agronomy, Hunan Agricultural University, Changsha 410128, P.R. China; 2. China Tobacco Hunan Industrial Co., Ltd., Changsha 410128, P.R. China
By introducing purslane DNA into tobacco via pollen-tube pathway, the 4th generation self-bred progeny (D4) was obtained, and the purity of 32 selected D4 were analyzed. The results showed that the optimum SRAP-PCR reaction system includes a solution...