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6 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 39

    The use of high content screening (HCS) in human primary lung cells to assess e-liquids of differing flavors and nicotine concentrations

    SIMMS L.(1); CZEKALA L.(1); STEVENSON M.(1); PHILLIPS G.(1); WALELE T.(2)
    (1) Imperial Brands, Bristol, U.K.; (2) Fontem Ventures, Amsterdam, The Netherlands
    Due to the current scientific debate and evolving regulatory landscape, new assays are needed to quickly determine the subtle biological response of e-liquids for both stewardship and product development purposes. For stewardship, all ingredients in the e ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 52

    Biological test procedure for fresh generated cigarette smoke and e-cigarette aerosols under air-liquid interface (ALI) exposure in 24 and 96 multi-well plates

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    Cigarette smoke and e cigarette aerosol contain particulate matter/liquid droplets in the solid phase as well as volatile substances in the gas phase. Both fractions are of potential toxicological relevance and should be considered when assessing ...
  3. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 53

    Use of human derived cell lines to increase the biological relevance of cigarette condensate, non-tobacco materials and e-liquids testing

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    The in vitro testing strategy of tobacco products is mainly based on the testing of extracts from cigarette smoke condensates. An accepted industry wide assay battery has been developed over recent years which delivers reliable results for the ...
  4. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, ST 48

    The proposed use of human cells to cover the ‘R’ end point for EUTPD2 data requirements from a 21st century toxicology perspective

    SIMMS L.(1); PALMER J.(2)
    (1) Imperial Tobacco Limited, Bristol, U.K.; (2) Stemina, Madison, WI, U.S.A.
    In 2007 the National Academies of Sciences released “Toxicity Testing in the 21st Century: A Vision and a Strategy”, a new toxicological paradigm, moving away from the idea of high dose animal tests as the ‘gold standard’ and focusing on the use of human ...
  5. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 32

    Cellular transformation activity of different tobacco condensates in the Bhas 42 cell assay

    STEVENSON M.(1); WIECZOREK R.(2); SIMMS L.(1); PANT K.(3)
    (1) Imperial Tobacco Limited, Bristol, U.K.; (2) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (3) BioReliance, Sigma-Aldrich Corp., Rockville, MD, U.S.A.
    Carcinogenesis has been described as a multi-stage process comprising of initiation, promotion and progression. Smoking is a cause of serious disease in smokers, including lung cancer. Determining the carcinogenic potential of a tobacco product ingredient ...
  6. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, ST 80

    The CORESTA recommended in vitro test battery alongside the National Research Council vision for toxicity testing in the 21st Century

    SIMMS L.(1); WIECZOREK R.(2); TRELLES STICKEN E.(2)
    (1) Imperial Tobacco Ltd, Bristol, U.K.; (2) Imperial Tobacco Group, Reemtsma Cigarettenfabriken GmbH, Biological Labs Hamburg, Hamburg, Germany
    The CORESTA In vitro Toxicity Testing of Tobacco Smoke Task Force recommended a test battery in 2004 which has been subsequently used by the tobacco industry. A similar in vitro test battery has been used by the International Community on Harmonisation ...