Search Results

14 results

  1. CORESTA Congress, Quebec, 2014, Agronomy/Phytopathology Groups, APPOST 03

    Agrobacterium T-DNA in Nicotiana tomentosiformis and other Nicotiana species

    CHEN K.(1); DORLHAC DE BORNE F.(2); SZEGEDI E.(3); OTTEN L.(1)
    (1) Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France; (2) National Agricultural Research and Innovation Centre (NARIC), Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, Hungary; (3) Imperial Tobacco Group, Institut du Tabac, Bergerac, France
    Agrobacterium rhizogenes induces growth of aberrant roots with bacterial T-DNA; some roots can spontaneously regenerate into plants carrying T-DNA fragments. T-DNAs contain genes that are expressed in plants which normally lead to root growth and to the ...
  2. CORESTA Congress, Edinburgh, 2010, SSPT 17

    GCxGC-MS characterisation of tobacco extracts: Classification and factors affecting the class maps

    VIAL J.; JOURNOUD P.; THIEBAUD A.; SASSIAT P.; TEILLET B.; CAHOURS X.; RIVALS I.
    ESPCI ParisTech, UMR CNRS UPMC PECSA, Laboratoire Sciences Analytiques, Bioanalytiques et Miniaturisation, Paris, France
    Comprehensive gas chromatography (GCxGC) appears as the preferred technique for the characterization of volatile compounds in tobacco samples. Indeed, GCxGC offers an enhanced separation power which results from the combination of two columns of different ...
  3. CORESTA Congress, Shanghai, 2008, SSPT 03

    Analysis of protein modifications induced by cigarette smoke

    LE FAOUDER P.; EMAMI I.; ROLANDO C.; TOKARSKI C.
    University of Sciences and Technologies of Lille, Laboratory of Organic and Macromolecular Chemistry, CNRS IMR 8009, Villeneuve d'Ascq, France
    We will present here work done on identification of protein modifications induced by cigarette smoke, particularly protein oxidation and nitration, using on-line nanoLC, ECD hyphenation on a FT-ICR instrument (Fourier Transform- Ion Cyclotron Resonance). ...
  4. CORESTA Congress, Shanghai, 2008, SSPT 07

    Identification and quantification of free radical content in cigarette smoke using nanoLC nano ESI FT-ICR MS/MS

    LE FAOUDER P.; TOKARSKI C.; EMAMI I.; ROLANDO C.
    University of Sciences and Technologies of Lille, Laboratory of Organic and Macromolecular Chemistry, CNRS IMR 8009, Villeneuve d'Ascq, France
    Cigarette smoke is a highly complex aerosol of several thousand chemical substances of which free radicals, short-life time molecules, are present in high concentration. In order to identify and quantify them, we have developed a new methodology based on ...
  5. CORESTA Congress, Shanghai, 2008, SSPTPOST 20

    Proteomics study of the influence of free radicals contents in cigarette smoke on culture cells

    LE FAOUDER P.; TOKARSKI C.; EMAMI I.; ROLANDO C.
    University of Sciences and Technologies of Lille, Laboratory of Organic and Macromolecular Chemistry, CNRS IMR 8009, Villeneuve d'Ascq, France
    The influence of free radicals from cigarette smoke on MCF-7 cell lines has been investigated in our laboratory using proteomics study. The work presented here will expose the comparison between untreated cells and cells incubated with conventional ...
  6. CORESTA Congress, Paris, 2006, PT 15

    Reconstituted tobacco treatment to reduce TSNA content of tobacco products - Part I: Tobacco treatment during reconstitution

    RIGOULAY C.; LE BEC L.; RAVERDY-LAMBERT D.; LE BOURVELLEC G.
    LTR Industries (SWM Intl.), Le Mans, France
    A lot of effort has been devoted since several years to reduce tobacco specific nitrosamines (TSNA) in tobacco products. Tobacco breeding, cultural and curing practices have been improved to prevent TSNA formation. The paper making reconstitution of ...
  7. CORESTA Congress, Paris, 2006, PT 16

    Reconstituted tobacco treatment to reduce TSNA content of tobacco products - Part II: Effect of the treatment on TSNA and Hoffmann analytes in mainstream smoke

    LE BEC L.; RIGOULAY C.; RAVERDY-LAMBERT D.; LE BOURVELLEC G.
    LTR Industries (SWM Intl.), Le Mans, France
    In part I, we have presented a treatment that reduces TSNA levels in tobacco during reconstitution and its effects on tobacco chemistry. The objective of this paper is to present the smoking results of the reconstituted tobacco treated to reduce the TSNA ...
  8. CORESTA Congress, Paris, 2006, PT 19

    Accelerated solvent extraction and supercritical fluid extraction coupled to comprehensive GCxGC for the analysis of volatile compounds in tobacco

    VIAL J.; THIEBAUT D.; SASSIAT P.; BELDEAN-GALEA M.S.; GOMEZ RAMOS M.J.; BOUZIGE M.
    ESPCI ParisTech, CNRS PECSA 7195, Laboratoire Environnement et Chimie Analytique, Paris, France
    Comprehensive GC×GC is a very powerful technique for complex sample analysis because a huge number of components can be separated according to carbon number and chemical classes. Two gas chromatography columns of different selectivities are connected via ...
  9. CORESTA Congress, Paris, 2006, SS 20

    Precise identification of free radicals in cigarette smoke by exact mass measurement using nano-LC, nanoESI FT-MS/MS

    ROLANDO C.; CANTAIS F.; EMAMI I.
    Université des Sciences et technologies de Lille, UMR CNRS 8009, Villeneuve d'Ascq cedex, France
    Free radicals are involved in a wide variety of ageing disease like cancer, cardiovascular disease and atherosclerosis. However, the molecular structure of free radicals that are contained in smoke is still the object of many controversies. This state ...
  10. CORESTA Congress, Paris, 2006, SS 33

    Proteomics study of the influence of cigarette smoke free radicals on culture cells

    ROLANDO C.; TOKARSKI C.; EMAMI I.
    Université des Sciences et technologies de Lille, UMR CNRS 8009, Villeneuve d'Ascq cedex, France
    The influence of cigarette smoke free radicals on cell lines has been investigated in vitro by many methods: Ames tests for detecting mutagenesis; DNA adducts formation and more recently gene expression using RNA arrays. To the best of our knowledge, ...