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6 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product. ...
  3. CORESTA Congress, Paris, 2006, PT 15

    Reconstituted tobacco treatment to reduce TSNA content of tobacco products - Part I: Tobacco treatment during reconstitution

    RIGOULAY C.; LE BEC L.; RAVERDY-LAMBERT D.; LE BOURVELLEC G.
    LTR Industries (SWM Intl.), Le Mans, France
    A lot of effort has been devoted since several years to reduce tobacco specific nitrosamines (TSNA) in tobacco products. Tobacco breeding, cultural and curing practices have been improved to prevent TSNA formation. The paper making reconstitution of ...
  4. CORESTA Congress, Paris, 2006, PT 16

    Reconstituted tobacco treatment to reduce TSNA content of tobacco products - Part II: Effect of the treatment on TSNA and Hoffmann analytes in mainstream smoke

    LE BEC L.; RIGOULAY C.; RAVERDY-LAMBERT D.; LE BOURVELLEC G.
    LTR Industries (SWM Intl.), Le Mans, France
    In part I, we have presented a treatment that reduces TSNA levels in tobacco during reconstitution and its effects on tobacco chemistry. The objective of this paper is to present the smoking results of the reconstituted tobacco treated to reduce the TSNA ...