CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 17
Using RNP CRISPR to create ultra-low nicotine tobacco
(1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. Our research showed that nicotine levels in PMT-RNAi and PR50-RNAi lines were reduced more than 95% and 80-90%,...