1. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 17

    Using RNP CRISPR to create ultra-low nicotine tobacco

    SHEN Y.(1); QI D.(2); YANG J.(1); KUDITHIPUDI C.(1); XU D.(1); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. Our research showed that nicotine levels in PMT-RNAi and PR50-RNAi lines were reduced more than 95% and 80-90%,...
  2. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 20

    Ultra-low nicotine tobacco lines with improved leaf quality

    KUDITHIPUDI C.(1); MORRIS J.W.(1); LUSSO M.(1); SHEN Y.(1); QI D.(2); DAVIS G.A.(1); LION K.(1); XU D.(1); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. The remaining fraction consists of three minor alkaloids: nornicotine, anabasine and anatabine. Genetic control...