1. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, AP 03

    Transcriptomic and metabolomic analysis of very low nicotine tobacco lines

    KUDITHIPUDI C.(1); SHEN Yanxin(1); GOVINDRAJULU R.(2); XU Dongmei(1); WAREK U.(1); STRICKLAND J.A.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, c/o Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90 % of total alkaloids. Recently, the U.S. Food and Drug Administration issued an advance notice of proposed rulemaking to obtain...
  2. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 04

    Pest and disease surveillance in flue-cured tobacco growing zones of India

    REDDY B.S.R.; SWAMY S.; MANI M.
    ITC Ltd, Agri Business Division, Research Department, Rajahmundry, A.P, India
    Pest and disease are major yield limiting factors in tobacco production and these biotic factors reduce the productivity besides affecting the quality of leaf. The control of these pest and diseases warrant more pesticide usage resulting in pesticide...
  3. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, ST 29 (also presented at TSRC 2018)

    Modification of standardized methods for the measurement of nicotine in very low nicotine content cigarettes

    BROWN A.P.(1); LIU Hui(2); MANSON K.(2); MELVIN M.S.(1); PIEROTTI J.(2); PITHAWALLA Y.B.(1); SMITH J.H.(1); STUTT K.(2); WAGNER K.A.(1)
    (1) Altria Client Services LLC, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Professional Scientific Staffing, Richmond, VA, U.S.A.
    In March 2018, the U.S. Food and Drug Administration (FDA) issued an advanced notice of proposed rulemaking (ANPRM) for a nicotine product standard that would limit the amount of nicotine in combusted tobacco products. FDA requested comments...
  4. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As...
  5. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product....
  6. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 15

    Fertigation of flue-cured tobacco with a combination of water soluble and straight fertilisers, for enhancing nitrogen and potassium use efficiency

    MAHESH M.N.V.A.; RAMAKRISHNA B.; MANI M.; SRINIVAS P.
    ITC Limited - Agri Business Division-ILTD, Research Department, Rajahmundry, Andhra Pradesh, India
    Nutrient application through drip irrigation (fertigation) with water soluble fertilisers, is an established practice to achieve nutrient use efficiency in flue-cured tobacco production. However, there has been a steady price escalation and erratic...
  7. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 17

    Using RNP CRISPR to create ultra-low nicotine tobacco

    SHEN Y.(1); QI D.(2); YANG J.(1); KUDITHIPUDI C.(1); XU D.(1); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. Our research showed that nicotine levels in PMT-RNAi and PR50-RNAi lines were reduced more than 95% and 80-90%,...
  8. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 20

    Ultra-low nicotine tobacco lines with improved leaf quality

    KUDITHIPUDI C.(1); MORRIS J.W.(1); LUSSO M.(1); SHEN Y.(1); QI D.(2); DAVIS G.A.(1); LION K.(1); XU D.(1); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. The remaining fraction consists of three minor alkaloids: nornicotine, anabasine and anatabine. Genetic control...
  9. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 65

    Management of Orobanche in flue-cured tobacco in India

    REDDY B.S.R.; SWAMY S.; MANI M.
    ITC Limited - Agri Business Division-ILTD, Research Department, Rajahmundry, Andhra Pradesh, India
    Orobanche (Orobanche cernua Loefl.) is a serious root parasite infesting tobacco roots in Northern Light Soil (NLS), Traditional and Mysore tobacco growing regions of India. The yield losses were ranging from 30-70% and plants show the symptoms of...
  10. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, ST 21 (also presented at TSRC 2016)

    Characterization of puff topography during 8-hours of ad libitum use of MarkTen® e-vapor products

    EDMISTON J.; LIANG Q.; VANSICKEL A.; LIU J.; ZHAO Y.; SARKAR M.
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Introduction: The purpose of this study was to characterize the puff topography variables following use of MarkTen® electronic cigarettes by adult conventional cigarette smokers [CS], adult cigarette and e-vapor dual users [DU] and adult exclusive...