1. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 04

    Pest and disease surveillance in flue-cured tobacco growing zones of India

    REDDY B.S.R.; SWAMY S.; MANI M.
    ITC Ltd, Agri Business Division, Research Department, Rajahmundry, A.P, India
    Pest and disease are major yield limiting factors in tobacco production and these biotic factors reduce the productivity besides affecting the quality of leaf. The control of these pest and diseases warrant more pesticide usage resulting in pesticide...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 12 (also presented at TSRC 2018)

    Characterization of the Vitrocell® high throughput exposure modules using different tobacco product types

    LEVERETTE R.(1); KEYSER B.(2); SEYMOUR A.(2); HOLLINGS M.(2); BOMBICK B.(1)
    (1) RAI Services Company, Scientific & Regulatory Affairs, Winston-Salem, NC, U.S.A.; (2) Covance Laboratories Ltd, Harrogate, North Yorkshire, U.K.
    The development of whole smoke/aerosol exposure systems provides a means to conduct in vitro assessment of freshly generated whole smoke and aerosol from combustible and tobacco heating products (THP) as well as electronic nicotine delivery systems...
  3. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 13 (also presented at TSRC 2018)

    Genotoxicity evaluation of tobacco and nicotine delivery products: Part 1. Mouse lymphoma assay

    LEVERETTE R.(1); THORNE D.(2); BREHENY D.(2); FOWLER K.(1); McENANEY S.(3); LLOYD M.(3); CLEMENTS J.(3); BOMBICK B.(1); GACA M.(2)
    (1) RAI Services Company, Scientific & Regulatory Affairs, Winston-Salem, NC, U.S.A.; (2) British American Tobacco (Investments) Ltd., Southampton, U.K.; (3) Covance Laboratories Ltd, Harrogate, North Yorkshire, U.K.
    In vitro studies have been widely used to support the toxicological evaluation of chemicals and complex mixtures including cigarette smoke. In this study a variety of test matrices from different tobacco and nicotine delivery products was assessed...
  4. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 14 (also presented at TSRC 2018)

    Genotoxicity evaluation of tobacco and nicotine delivery products: Part 2. In Vitro micronucleus assay

    LEVERETTE R.(1); THORNE D.(2); BREHENY D.(2); FOWLER K.(1); McENANEY S.(3); WHITWELL J.(3); CLEMENTS J.(3); BOMBICK B.(1); GACA M.(2)
    (1) RAI Services Company, Scientific & Regulatory Affairs, Winston-Salem, NC, U.S.A.; (2) British American Tobacco (Investments) Ltd., Southampton, U.K.; (3) Covance Laboratories Ltd, Harrogate, North Yorkshire, U.K.
    In vitro studies have been widely used to support toxicological evaluations of chemicals and complex mixtures including cigarette smoke. In this study a variety of test matrices from tobacco and nicotine delivery products were assessed against a 3R4F...
  5. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 26

    Determination of carbonyl compounds in gas vapour phase in phosphate buffered saline by LC-MS/MS for use with traditional tobacco and electronic nicotine delivery system (ENDS) products

    HOLLINGS M.(1); SEYMOUR A.(1); LYNCH A.(2); LARNER J.(1); CLEMENTS J.(1); HENDERSON L.(2)
    (1) Covance Laboratories Limited, Harrogate, U.K.; (2) Broughton Laboratories Ltd, Skipton, U.K.
    The purpose of this study was to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to quantify selected carbonyl compounds (formaldehyde, acetaldehyde, crotonaldehyde, acrolein, acetone, butyraldehyde, 2-butanone (MEK)...
  6. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 51

    Simultaneous measurement of cilia beat frequency, goblet cell hyperplasia and TEER endpoints in EpiAirway™-FT treated tissues may reveal underlying toxicity mechanisms in vitro

    WOODHAMS A.(1); KIDD D.(1); DAUNT A.(2); POSTOYALKO A.(2); HOLLINGS M.(1)
    (1) Department of Genetic Toxicology, Covance Laboratories Ltd, Harrogate, U.K.; (2) Special Pathology Services, Covance Laboratories Limited, Harrogate, U.K.
    The EpiAirway™-FT (full thickness) tissues (Mattek Corporation, Ashland, USA) contain tight junctions, ciliated and other bronchial cell types making it an ideal model for testing aerosolised substances in vitro. Toxicity is not a single endpoint but...
  7. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 15

    Fertigation of flue-cured tobacco with a combination of water soluble and straight fertilisers, for enhancing nitrogen and potassium use efficiency

    MAHESH M.N.V.A.; RAMAKRISHNA B.; MANI M.; SRINIVAS P.
    ITC Limited - Agri Business Division-ILTD, Research Department, Rajahmundry, Andhra Pradesh, India
    Nutrient application through drip irrigation (fertigation) with water soluble fertilisers, is an established practice to achieve nutrient use efficiency in flue-cured tobacco production. However, there has been a steady price escalation and erratic...
  8. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 65

    Management of Orobanche in flue-cured tobacco in India

    REDDY B.S.R.; SWAMY S.; MANI M.
    ITC Limited - Agri Business Division-ILTD, Research Department, Rajahmundry, Andhra Pradesh, India
    Orobanche (Orobanche cernua Loefl.) is a serious root parasite infesting tobacco roots in Northern Light Soil (NLS), Traditional and Mysore tobacco growing regions of India. The yield losses were ranging from 30-70% and plants show the symptoms of...
  9. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 26

    A review of aerosol exposure systems relative to the analysis of cytotoxicity: a CORESTA In vitro Toxicity Testing Sub-Group perspective

    THORNE D.(1); WIECZOREK R.(2); FUKUSHIMA T.(3); SHIN H.J.(4); LEVERETTE R.(5); BALLANTYNE M.(6); LI X.(7); BOMBICK B.(5)
    (1) British American Tobacco, R&D Centre, Southampton, U.K.; (2) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (3) Japan Tobacco Inc., Scientific Product Assessment Centre, Yokohama, Kanagawa, Japan; (4) KT&G Corporation, Daejeon, South Korea; (5) RAI Services Company, Winston-Salem, NC, U.S.A.; (6) Covance Laboratories Ltd, Harrogate, U.K.; (7) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, P.R. China
    Aerosol exposure systems offer researchers a variety of ways to customise the exposure set-up, modify experimental parameters and provide a novel and versatile platform for in vitro aerosol research. These systems produce an aerosol that more closely...
  10. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 34

    The mutagenic assessment of electronic-cigarettes and tobacco smoke using the Ames assay in strains TA98 and TA100

    THORNE D.(1); CROOKS I.(1); HOLLINGS M.(2); SEYMOUR A.(2); MEREDITH C.(1); GAÇA M.D.(1)
    (1) British American Tobacco, R&D Centre, Southampton, U.K.; (2) Covance Laboratories Ltd, Harrogate, U.K.
    Salmonella typhimurium strains TA98 and TA100 were used to assess the mutagenic potential of a commercially available rechargeable, dual voltage, closed system modular electronic-cigarette (Vype® ePen, Nicoventures UK). Results were compared to a...