Search Results

6 results

  1. TSRC, Tob. Sci. Res. Conf., 2016, 70, abstr. 17

    Ex vivo effects of smokeless tobacco extracts on select immune responses

    PRASAD G.L.(1); ARIMILLI S.(2); DAMRATOSKI B.E.(2); SCHMIDT E.(1); ROWE J.(3); CHOUCHANE S.(4)
    (1) RAI Services Company; (2) Wake Forest University Health Sciences; (3) R.J. Reynolds Tobacco Co.; (4) Eurofins Lancaster Labs Inc, Winston-Salem, NC, USA
    Our previous work demonstrated that exposure to Tobacco Product Preparations (TPPs) from combustible tobacco cause cytotoxic and select immunosuppressive effects in PBMCs, and this ex vivo model is well suited to investigate the chronic immune suppression ...
  2. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 19

    Tobacco product preparations differentially regulate human PBMC functions, including T cell and NK function.

    ARIMILLI S.(1); DAMRATOSKI B.E.(1); PRASAD G.L.(2)
    (1) Wake Forest University School of Medicine, Winston-Salem, NC, USA; (2) Reynolds Tobacco Company, Winston-Salem, NC, USA
    Natural Killer (NK) cells and T cells play essential roles in innate and adaptive immune responses in protecting against microbial infections and in tumor surveillance. Although evidence suggests that smoking causes immunosuppression, there is limited ...
  3. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 05

    A cell culture model for chronic exposure to cigarette smoke.

    ARIMILLI S.(1); DAMRATOSKI B.E.(1); PRASAD G.L.(2)
    (1) Dept. of Microbiology & Immunology, Wake Forest University School of Medicine, Winston Salem, NC, USA; (2) R.J. Reynolds Tobacco Company, Winston Salem, NC, USA
    A wide range of experimental models, including cell culture systems, have been developed to investigate the cytotoxic and biological effects of cigarette smoke and its constituents, individually and by chemical class. For example, cell culture studies ...
  4. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 38

    Cytotoxic effects of different tobacco preparations on human peripheral blood mononeucleocytes.

    ARIMILLI S.(1); DAMRATOSKI B.E.(1); PRASAD G.L.(2)
    (1) Dept. of Microbiology & Immunology, Wake Forest University School of Medicine, Winston Salem, NC, USA; (2) R.J. Reynolds Tobacco Company, Winston Salem, NC, USA
    Acute exposure to cigarette smoke or its components has been known to trigger diverse cellular effects, such as oxidative stress, DNA damage and cell death in a number of cell culture models. However, available data regarding the potential cytotoxic ...
  5. TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 19

    Optimization of leukocyte isolation for clinical studies

    ARIMILLI S.; DAMRATOSKI B.E.; CHEN P.; JONES B.A.; SWORDS W.E.; PRASAD G.L.
    Wake Forest University School of Medicine, Dept. of Microbiology & Immunology, Winston-Salem, NC, USA; R.J. Reynolds Tobacco Co., IR&D Dept., Winston-Salem, NC, USA
    Isolation and processing blood into its constituent cell types is an important process in basic and clinical research. Although a number of reagents are available and methods have been developed to fractionate blood, there is a need to optimize methods ...
  6. TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 22

    Evaluation of cytotoxicity of different tobacco preparations

    ARIMILLI S.; DAMRATOSKI B.E.; BOMBICK B.; SWORDS W.E.; BORGERDING M.; PRASAD G.L.
    Wake Forest University School of Medicine, Dept. of Microbiology & Immunology, Winston-Salem, NC, USA
    Current debate regarding the potential for significant reductions in human health risk associated with smokeless tobacco (ST) use compared to cigarette smoking suggests further examination of the relative cellular/molecular effects of non-combustible and ...