Search Results

3 results

  1. TSRC, Tob. Sci. Res. Conf., 2014, 68, abstr. 30

    Application of TILLING technology to screen for mutations in the tobacco glycosyltransferase gene NtGt1a

    FU Qiang; ZOU Jie; ZHANG Jishun; LIN Shifeng; YU Jing; GUO Yushuang; ZHANG Xiaolian; YU Shizhou; ZHAO Jiehong; REN Xueliang
    Guizhou Academy of Tobacco Science, Guiyang, Guizhou, China
    Targeting Induced Local Lesions IN Genomes (TILLING) combines chemical mutagenesis with high throughput screening to allow for the generation and detection of mutant alleles in selected genes. In this study, TILLING has been applied to produce a series of ...
  2. TSRC, Tob. Sci. Res. Conf., 2014, 68, abstr. 90

    Isolation and functional analysis of an ltr-retrotransposon from tobacco (Nicotiana tabacum)

    GUO Yushuang; LIN Shifeng; YU Jing; ZOU Jie; FU Qiang; YU Shizhou; ZHANG Xiaolian; ZHAO Jiehong; REN Xueliang
    Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Sciences, Guiyang, China
    Long terminal repeat (LTR)-retrotransposons are genetic elements that can move and sometimes spread within genomes, and are important for plant genomic organization. In this research, a novel LTR-retrotransposon gene named Ntrt1 was isolated from ...
  3. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 86

    Genome-wide selection and identification of suitable reference genes for quantitative gene expression studies in tobacco (Nicotiana tabacum).

    GUO Yushuang; LIE Ruiyuan; CHEN Qingyuan; YANG Zhixiao; WANG Yi; ZHAO Jiehong; REN Xueliang
    Guizhou Academy of Tobacco Science, Guiyang City, P.R. China
    The real time quantitative reverse transcription PCR (qRT-PCR) is becoming increasingly important to measure gene expression levels. Accurate and reproducible results are dependent on the correct choice of the reference genes for data normalization. ...