Search Results

13 results

  1. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 111 (also presented at CORESTA 2018)

    Characterization and certification of smokeless tobacco reference products

    SLONE S.; CANETE S.; JI Huihua; JACK A.M.; BUSH L.P.; CHAMBERS O.; SHELTON B.
    University of Kentucky, Lexington, KY, USA
    The constituents of raw tobacco products are naturally variable, and product design, the manufacturing process and the evolving science of tobacco analytics may increase this variability. The call for certified tobacco reference materials and their ...
  2. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 43

    The pattern of benzo[a]pyrene and tobacco-specific nitrosamine accumulation in fire-cured tobacco

    JACK A.M.; BAILEY W.A.; SCHOERGENDORFER A.; JI H.; SINGLETON J.; FANNIN N.; BUSH L.P.
    University of Kentucky, Lexington, KY, USA
    The accumulation of benzo[a]pyrene (B[a]P) and tobacco specific nitrosamines (TSNAs) during fire-curing was studied in order to establish whether B[a]P and TSNAs can be reduced by modifying the firing regime. The dark fire-cured variety PD 7309 was grown ...
  3. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 13

    Accumulation of nicotine and nornicotine enantiomers in leaf of nicotine demethylases mutants during growth and curing

    CAI B.(1); JACK A.M.(1); LEWIS R.S.(2); DEWEY R.E.(2); JI H.(1); BUSH L.P.(1)
    (1) University of Kentucky, Lexington, KY, USA; (2) North Carolina State University, Raleigh, NC, USA
    Nicotine metabolism in tobacco plants is mostly through N-demethylation, resulting in nornicotine. There are three functional nicotine demethylases CYP82E4 (E4), CYP82E5v2 (E5) and CYP82E10 (E10) in tobacco. In this study, we investigated the nicotine and ...
  4. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 35

    The effect of harvest maturity on tsna accumulation in burley tobacco – different approaches

    JACK A.M.; FISHER C.R.; SCHOERGENDORFER A.; JI H.; FANNIN N.F.; BUSH L.P.
    University of Kentucky, Lexington, KY, USA
    The accumulation of tobacco specific nitrosamines (TSNAs) in burley tobacco is affected by a number of interacting factors. Previous work by several researchers has indicated an initial increase in TSNA accumulation with increasing maturity at harvest, ...
  5. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 57

    Influence of nitrogen fertilization rate and alleles at the yellow burley loci on TSNA formation in air-cured tobacco.

    LEWIS R.S.(1); PARKER R.(1); JACK A.M.(2); DANEHOWER D.(1); WHITLEY S.(1); BUSH L.P.(2)
    (1) Crop Science Dept., North Carolina State University, Raleigh, NC, USA; (2) University of Kentucky, Lexington, KY, USA
    TSNA formation is influenced by alkaloid levels and the availability of nitrosating agents. Tobacco types differ in their potential for TSNA accumulation due to genetic and agronomic factors. Highest TSNA concentrations are typically exhibited by burley ...
  6. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 59

    Contributions of three nicotine demethylases to nicotine and nornicotine composition in the plant.

    CAI B.(1); FANNIN F.(1); LEWIS R.S.(2); DEWEY R.(2); BUSH L.P.(1)
    (1) Dept of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA; (2) Dept of Crop Science, NC State University, Raleigh, NC, USA
    Nicotine is synthesized in the tobacco root and translocated to the leaf where it may be demethylated to nornicotine. Three functional nicotine demethylases (CYP82E4, CYP82E5 and CYP82E10) have been identified in tobacco to date. CYP82E4 (E4) is the major ...
  7. CORESTA Congress, Edinburgh, 2010, AP 01; TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 45

    Nicotine is enantioselectively demethylated by cytochrome P450 enzyme CYP82E4

    CAI B.; FANNIN F.F.; CHAPPELL J.; BUSH L.P.
    University of Kentucky, Dept. of Plant & Soil Sciences, Lexington, KY, USA
    Nicotine and nornicotine are two of the dominant alkaloids found in tobacco (N. tabacum L.), and known precursors to 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN).  In tobacco nicotine is N-demethylated to ...