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  1. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 09

    Using direct PCR method of hyphae for ISSR Marker and ITS sequence study of Phytophthora nicotianae

    LEI Liping(1); QIN Xiyun(1); WAN Anyun(1); CHAI Jiarong(1); XIA Zhenyuan(1); CHEN Hairu(2); YANG Genhua(2)
    (1) Yunnan Academy of Tobacco Sciences, Yuxi, Yunnan Province 653100, P.R China; (2) Phytopathology Laboratory of Yunnan Province, Yunnan Agricultural University, Kunming 650201, P.R. China
    To investigate the diversity of Phytophthora nicotianae in the Yunnan Province of China, the direct PCR method was used to compare the differences in inter-translation space (ITS) of ribosomal DNA of Phytophthora nicotianae between the isolates from...
  2. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 13

    Effects of humic acids on the accumulation in tobacco leaves of lead (Pb) and cadmium (Cd) found in different soils

    ZHANG Yanling(1); YANG Xin(2); ZHANG Shixiang(1); TIAN Yangyang(1)
    (1) Zhengzhou 450001 Tobacco Research Institute of CNTC, Zhengzhou 450001, Henan, P.R China; (2) P.R China Tobacco Guangdong Industrial Corporation, P.R China
    Lead (Pb) and cadmium (Cd) are two harmful heavy metals in tobacco leaf that mainly come from soil. To explore a method to reduce lead and cadmium in tobacco leaf, pot cultivation with unpolluted and polluted soil was executed with four main tobacco...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 15

    Nitrogen supplying capacity of field soil under long-term tobacco-rice rotation and grassplot

    YI Jian-hua; PENG Yu; ZHOU Shu-guang; WANG Xiu-zhong; PENG Xin-hui; ZHOU Yan; ZHANG Wen-li
    Technology Center of P.R China Tobacco Hunan Industry Co. Ltd., Changsha, P.R China
    Field and greenhouse incubation experiments with 15N labelling were conducted to explore the effects of long-term tobacco-rice rotation practice on the characteristics of soil nitrogen (N) mineralization. The results showed that, related to the...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 26

    Development and application of Immunocapture real-time RT-PCR for detection of potato virus Y (PVY) in individual peach aphid (Myzus persicae)

    CHEN Dexin(1); YANG Jinguang(1); WANG Fenglong(1); ZHOU Wenliang(2); SHEN Lili(1); QIAN Yumei(1)
    (1) Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Qingdao, Shandong 266101, P.R.China; (2) Guangxi Tobacco Company Baise Branch, Baise 533000, P.R. China
    Potato virus Y (PVY) is transmitted in a nonpersistent manner by peach aphid (Myzus persicae), leading to many crop diseases. PVY infected tobacco plants could cause serious damage on tobacco quality and yields due to its simple transmission path and...
  6. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 05

    Cloning and gene structure analysis of T-psy1 from flue-cured tobacco

    CAI Liuti(1); HU Zhongyi(1); LEI Lu(1); LI Jixin(2)
    (1) Guizhou Tobacco Research Institute, Guizhou Provincial Tobacco Company, Guiyang, P.R China; (2) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Carotenoid is an important precursor of tobacco aroma, and phytoene synthase is one of the key enzymes in the pathway of aroma precursors biosynthesis and accumulation in tobacco. Based on the homology cloning method, the cDNA and DNA sequence of the...
  7. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 07

    Comparative transcriptome of tobacco leaves in typical ecological areas of Guizhou

    LEI Bo(1); PAN Wenjie(1); DING Fuzhang(1); ZHANG Kai(2); LI Jinxin(3); LU Ku(2)
    (1) Guizhou Tobacco Research Institute, Guiyang, P.R China; (2) College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R China; (3) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Flue-cured tobacco (Nicotiana tabacum) is a sensitive crop to ecological environment, and distinctive tobacco has always formed under special ecological conditions. In order to investigate the molecular mechanism of formation of medium and light...
  8. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 08

    Clone of glycine-rich RNA-binding proteins genes from different tobacco germplasm resources and expression analysis under different abiotic stresses

    ZHANG Qi(1); CHEN Jing-yun(1); LI Wen-zheng(2); CHEN Xuan(1); LIU Jian-ming(1)
    (1) Shanxi Kunming Tobacco Co. Ltd, Taiyua, P.R China; (2) Yunnan Tobacco Research Institute, Yuxi, P.R China
    Glycine-rich RNA-binding proteins (GR-RBPs) play important roles in the post-transcriptional regulation. In this paper, 11 GR-RBPs genes (RGP-3) were isolated and sequenced from different tobacco varieties including cultivated tobacco and wild...
  9. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, PPOST 03

    The application of resistance signal transfer by grafting against TMV in Nicotiana tabacum

    WANG Fenglong(1); YANG Jinguang(1); ZHAI Xilun(1); SONG Liyun(1); SHEN Lili(1); QIAN Yumei(1); HUANG Jin(2); WANG Yong(3)
    (1) Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, STMB, Tobacco Research Institute of CAAS, Qingdao 266101, P.R China; (2) Tobacco Corporation of Guangxi Baise, Baise 533000, P.R.China; (3) China Tobacco Shandong Industrial Co. Ltd., Jinan 250100, P.R. China
    The tobacco virus disease caused by tobacco mosaic virus (TMV) is one of the most important diseases of tobacco in China, and no effective prevention methods are used to control the TMV virus diseases. In this study, Nicotiana tabacum var. Samsun NN...
  10. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, PPOST 04

    Molecular variation characterization of the genome 5' terminal region of PVY isolated from tobacco in China

    YANG Jun; LUO Zhao-peng; JIN Li-feng; ZHAI Niu; LI Feng; XU Ya-long; ZHOU Hui-na; LIN Fu-cheng
    Zhengzhou Tobacco Research Institute, Zhegzhou, Henan, P.R. China.
    Tobacco viral diseases, of which most are RNA viruses, are a category of infectious diseases that are distributed widely and occur severely. The natural variation (mutation) of tobacco viruses proceed quickly, and cause considerable difficulty for...