TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 26
Single-nucleotide polymorphism frequency between Nicotiana tabacum cultivars.
Dept. of Industrial Plant Science & Technology, Chungbuk National University, Chungbuk Cheongju, South Korea
We analyzed 163,000 genomic DNA sequences downloaded from Tobacco Genome Initiative database and assembled to 31,370 contigs and 60,000 singletons. Using relatively long contigs, we designed primer sets for PCR amplification. We amplified 61 loci...