Search Results

8 results

  1. CORESTA Meeting, Agronomy/Phytopathology, 2017, Santa Cruz do Sul, AP 53

    Interactions between tobacco bushy top virus and its satellite RNA

    MO Xiaohan(1); ZHANG Lifang(1,2); ZHAO Xingneng(1,2); XU Ping(1,2); LI Yanqiong(1,2); XIA Zhenyuan(1); QIN Xiyun(1); CHEN Hairu(2)
    (1) Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China; (2) College of Plant Protection, Yunnan Agricultural University, Kunming, P.R. China
    Tobacco bushy top disease (TBTD) has caused significant yield and economic losses in Yunnan Province, China. The disease is caused by an unique virus complex consisting of tobacco bushy top virus (TBTV), TBTV satellite RNA, tobacco vein distorting...
  2. TSRC, Tob. Sci. Res. Conf., 2017, 71, abstr. 123

    The regulation of microorganism on the formation of TSNAs in tobacco

    ZOU Congming; XIA Zhenyuan; LEI Liping; HUANG Wei; ZHAO Gaokun; JIN Yan; WU Qun
    Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China
    TSNAs in tobacco are directly approved to be the carcinogens which is getting more and more attention than before. The regulation of TSNAs is becoming one of the most important topics of tobacco harm reduction. It has been shown that TSNAs formation...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, AP 03

    The interactions between tobacco vein distorting virus and tobacco bushy top disease-associated RNA

    MO Xiaohan; XU Ping; ZHAO Xingneng; ZHANG Lifang; QIN Xiyun; XIA Zhenyuan
    Yunnan Academy of Tobacco Agricultural Sciences of CNTC, Kunming, Yunnan, P.R. China
    Tobacco bushy top disease is the only plant viral disease caused by the umbravirus/luteovirus complex so far in China, resulting in significant economic losses in tobacco production in the Yunnan Province of China. Tobacco bushy top disease complex...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 09

    Using direct PCR method of hyphae for ISSR Marker and ITS sequence study of Phytophthora nicotianae

    LEI Liping(1); QIN Xiyun(1); WAN Anyun(1); CHAI Jiarong(1); XIA Zhenyuan(1); CHEN Hairu(2); YANG Genhua(2)
    (1) Yunnan Academy of Tobacco Sciences, Yuxi, Yunnan Province 653100, P.R China; (2) Phytopathology Laboratory of Yunnan Province, Yunnan Agricultural University, Kunming 650201, P.R. China
    To investigate the diversity of Phytophthora nicotianae in the Yunnan Province of China, the direct PCR method was used to compare the differences in inter-translation space (ITS) of ribosomal DNA of Phytophthora nicotianae between the isolates from...
  5. CORESTA Meeting, Agronomy/Phytopathology, Rovinj, 2009, APPOST 18

    Molecular characterization of the panB gene in a nicotine-degrading strain of Pseudomonas putida

    LEI Liping; WEI Hailei; XIA Zhenyuan; LIU Shuo; LIU Xingzhong
    Yunnan Tobacco Science Research Institute, Yuxi, Yunnan, China.
    A nicotine-sensitive mutant was generated from the nicotine-degrading bacterium, Pseudomonas putida strain J5, by mini-Tn5 transposon mutagenesis. This mutant was unable to grow with nicotine as the sole carbon source but could grow with glucose....
  6. CORESTA Meeting, Agronomy/Phytopathology, Krakow, 2007, AP 12

    Isolation and characterization of nicotine-degrading bacterial strain L1

    LEI Liping; XIA Zhenyuan; WANG Yue; WEI Hailei; LIU Xingzhong
    Yunnan Tobacco Science Research Institute, Yuxi, Yunnan, China.
    A bacterial strain L1, capable of degrading 1.5 g/l nicotine, was isolated from tobacco leaves. It was identified as a member of Bacillus simplex based on morphology, physiological and chemical tests, 16S rDNA sequence and phylogenetic...
  7. CORESTA Congress, Paris, 2006, AP 26

    Specific molecular markers of biocontrol fungus Pochonia chlamydospora ZK7 for tobacco root-knot nematode

    ZHU Mingliang; XIA Zhenyuan; YANG Shujun; LI Yunhua; ZHANG Keqin
    Yunnan Tobacco Science Research Institute, Yuxi, Yunnan, China.
    Biocontrol fungus Pochonia chlamydospora ZK7 are highly effective in the biological control against root-knot nematodes infecting tobacco. To obtain the specific molecular marker of P. chlamydospora ZK7, distinct and reproducible molecular...