Search Results

12 results

  1. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 54

    The regulation of autophagy of susceptible Nicotiana tabacum under tobacco mosaic virus infection

    YANG Jinguang(1); LIU Xaingfu(1); LIU Wei(1); QIAN Yumei(1); ZHANG Jing(2); LI Wei(2); DONG Shifei(2); WANG Yaofeng(3); HUANG Mingdi(4); WANG Fenglong(1)
    (1) Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Qingdao, P.R. China; (2) Hongyun Honghe Tobacco (Group) Co., Ltd., Kunming, P.R. China; (3) Qingyang Branch of Gansu Province Tobacco Company, Xifeng, P.R. China; (4) Longnan Tobacco Company, Gansu Tobacco Cooperation, Wudu 746000, P.R. China
    Autophagy (or self-eating) is an important conserved process by which eukaryotic cells recycle intracellular components. This study was undertaken to elucidate whether tobacco mosaic virus (TMV) infection on tobacco plants induced autophagy. TMV-U1...
  2. CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, AP 04

    Selection and identification of a tobacco endophytic bacteria strain L1 with inhibitory effect against TMV

    ZHAN Huaixu; WANG Fenglong; SHEN Lili; YANG Jinguang; QIAN Yumei; CHEN Dexin
    Qingzhou Tobacco Research Institute of CNTC, Qingdao, Shandong, P.R. China
    The objective of this study is to screen a biocontrol bacterial strain with significant inhibitory effect against TMV (Tobacco mosaic virus). The strains were isolated from leaves of tobacco var. Luyan 1 resistant to TMV. The anti-TMV strain was...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, AP 10

    Biological control of Ralstonia solanacearum using Ralstonia phage and the influence of diversity of soil bacteria

    WANG Fenglong(1); GAO Miao(1); QIAN Yumei(1); SHEN Lili(1); YU Guanghong(2); WANG Yaofeng(3); YANG Jinguang(1)
    (1) Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management / Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, P.R. China; (2) Baoshan Oriental Tobacco Company Ltd, Baoshan, Yunnan, P.R. China; (3) Qingyang Company, Gansu Provincial Tobacco, Xifeng, Gansu, P.R. China
    Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the main diseases of tobacco. Ralstonia phage ∈RS-1 was isolated from the soil of tobacco bacterial wilt nurseries from experimental fields of the Tobacco Research Institute of CAAS...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 26

    Development and application of Immunocapture real-time RT-PCR for detection of potato virus Y (PVY) in individual peach aphid (Myzus persicae)

    CHEN Dexin(1); YANG Jinguang(1); WANG Fenglong(1); ZHOU Wenliang(2); SHEN Lili(1); QIAN Yumei(1)
    (1) Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Qingdao, Shandong 266101, P.R.China; (2) Guangxi Tobacco Company Baise Branch, Baise 533000, P.R. China
    Potato virus Y (PVY) is transmitted in a nonpersistent manner by peach aphid (Myzus persicae), leading to many crop diseases. PVY infected tobacco plants could cause serious damage on tobacco quality and yields due to its simple transmission path and...
  5. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, PPOST 03

    The application of resistance signal transfer by grafting against TMV in Nicotiana tabacum

    WANG Fenglong(1); YANG Jinguang(1); ZHAI Xilun(1); SONG Liyun(1); SHEN Lili(1); QIAN Yumei(1); HUANG Jin(2); WANG Yong(3)
    (1) Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, STMB, Tobacco Research Institute of CAAS, Qingdao 266101, P.R China; (2) Tobacco Corporation of Guangxi Baise, Baise 533000, P.R.China; (3) China Tobacco Shandong Industrial Co. Ltd., Jinan 250100, P.R. China
    The tobacco virus disease caused by tobacco mosaic virus (TMV) is one of the most important diseases of tobacco in China, and no effective prevention methods are used to control the TMV virus diseases. In this study, Nicotiana tabacum var. Samsun NN...
  6. CORESTA Congress, Edinburgh, 2010, AP 09

    Establishment and application of multiple RT-PCR for detection of TMV, CMV and PVY in tobacco

    CHEN Dexin; YANG Jinguang; ZHANG Shuai; SHEN Lili; QIAN Yumei; WANG Changshuan; HUANG Jin; WANG Fenglong
    Chinese Academy of Agricultural Science, Key Lab. of Tobacco Pest Monitoring Controlling & Integrated Mgt, STMB, Tobacco Research Inst., Qingdao, China
    The sequences of tobacco mosaic virus (TMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) in the GenBank database were aligned using DNAMAN 6.0 version software, and the primers were designed on the conserved regions. And the multiple RT-PCR...
  7. CORESTA Meeting, Agronomy/Phytopathology, Rovinj, 2009, AP 01

    Rapid diagnosis of tobacco mosaic virus using the loop-mediated isothermal amplification method (LAMP)

    LIU Yanhua; WANG Zhide; QIAN Yumei; MU Jianmin; SHEN Lili; ZHANG Xingwei
    Qingzhou Tobacco Research Institute of CNTC, Qingzhou, Shandong, China
    Tobacco mosaic virus (TMV) is a severe worldwide disease and capable of greatly reducing tobacco quality and yield. In this study, we developed the loop-mediated isothermal amplification (LAMP) method for the specific detection of this virus. Mg2+...
  8. CORESTA Meeting, Agronomy/Phytopathology, Rovinj, 2009, APPOST 21

    A real-time reverse transcription PCR assay for detection of Cucumber mosaic virus in individual peach aphid Myzus persicae

    WANG Fenglong; YANG Jinguang; WANG Fang; SHEN Lili; QIAN Yumei
    Qingzhou Tobacco Research Institute of CNTC, Qingzhou, Shandong, China, and Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, Shandong, China
    Cucumber mosaic virus (CMV) is transmitted in a nonpersistent manner by peach aphid ( Myzus persicae ), leading to many crop diseases. The large number of viruliferous peach M. persicae is one of the major culprits for the rampant outbreak. A...
  9. CORESTA Congress, Shanghai, 2008, AP 30

    The study on extracting antivirus protein of an antagonistic bacteria and action mode resistance to TMV

    SHEN Lili; WANG Fenglong; QIAN Yumei; CHEN Dexin; WU Huihui; HE Jingmei
    Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China
    In the laboratory, we found that the TMV contaminated by bacillus decreased the ability of infection and the symptom on the inoculated tobacco was gentle. So we separated an antivirus bacteria resistance to TMV in tobacco fields, which we named By33....
  10. CORESTA Meeting, Agronomy/Phytopathology, Santa Cruz do Sul, 2005, PPOST 09

    Dynamic changes of catalase during the interaction of tobacco and cucumber mosaic virus

    WANG Fenglong; WEI Yingying; QIAN Yumei; CHEN Dexin
    Tobacco Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
    The infection of virus will induce the accumulation of active oxygen species (AOS) in plants, which is a kind of spectrum resistance. But on the other side, active oxygen species can damage the plants, thus there are active oxygen species eliminating...