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  2. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 16

    Role of polyamines in leaf ripening of low alkaloid tobacco varieties

    NÖLKE G.(1); VOLKE D.(1); CHUDOBOVA I.(1); HOUDELET M.(1); LUSSO M.(2); FREDERICK J.(2); WAREK U.(2); STRICKLAND J.(2); XU D.(2); FISCHER R.(1); SCHINKEL H.(1); SCHILLBERG S.(1)
    (1) Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany; (2) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.
    Traditional breeding and molecular approaches have been used to develop tobacco varieties with reduced nicotine and secondary alkaloid levels. However, low-alkaloid (LA) tobacco varieties often show impaired leaf ripening and senescence leading to...
  3. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 17

    Using RNP CRISPR to create ultra-low nicotine tobacco

    SHEN Y.(1); QI D.(2); YANG J.(1); KUDITHIPUDI C.(1); XU D.(1); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. Our research showed that nicotine levels in PMT-RNAi and PR50-RNAi lines were reduced more than 95% and 80-90%,...
  4. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 18

    Reduction of nicotine content in tobacco leaves by modification of transporter gene expression

    FREDERICK J.; XU D.; DAVIS G.A.; LION K.; WAREK U.; STRICKLAND J.
    Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.
    Current tobacco lines with reduced nicotine content derived from nic1nic2 mutants have inferior leaf quality and undesirable smoking characteristics possibly due to the metabolic consequences of blocked nicotine biosynthesis. Allowing root production...
  5. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 20

    Ultra-low nicotine tobacco lines with improved leaf quality

    KUDITHIPUDI C.(1); MORRIS J.W.(1); LUSSO M.(1); SHEN Y.(1); QI D.(2); DAVIS G.A.(1); LION K.(1); XU D.(1); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Eurofins Lancaster Laboratories, Lancaster, PA, c/o Altria Client Services LLC, Richmond, VA, U.S.A.
    Nicotine is the most abundant alkaloid in cultivated tobacco (Nicotiana tabacum), typically constituting more than 90% of total alkaloids. The remaining fraction consists of three minor alkaloids: nornicotine, anabasine and anatabine. Genetic control...
  6. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 23

    Tobacco genome sequence and its applications to genome-based selection

    XU D.; FREDERICK J.; ADAMS A.; PRAMOD S.; KUDITHIPUDI C.; SHEN Y.; LUSSO M.; WAREK U.; STRICKLAND J.
    Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.
    Tobacco (Nicotiana tabacum L.), a model organism for research, is also an economically important crop. Tobacco is an allotetraploid derived from interspecific hybridization involving the two diploid species Nicotiana sylvestris and Nicotiana...
  7. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 24

    Characterization of nitrogen use efficient tobacco for molecular marker development

    FREDERICK J.; KUDITHIPUDI C.; XU D.; WAREK U.; STRICKLAND J.
    Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.
    High rates of nitrogen fertilization are required currently for cultivation of Burley tobacco to achieve yields and qualities that are desired. However, Maryland type tobaccos are grown using a nitrogen fertilization rate that is ~60-70% lower than...
  8. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 25

    Sucker control through biotechnology

    XU D.(1); GRUNDMANN L.(2); SHEN Y.(1); NOLL G.(2); YANG J.(1); KUDITHIPUDI C.(1); FREDERICK J.(1); PRUEFER D.(2); WAREK U.(1); STRICKLAND J.(1)
    (1) Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.; (2) Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Muenster, Germany
    Tobacco plants exhibit strong apical dominance that effectively inhibits axillary bud development. However, upon shoot apical meristem removal (topping), loss of apical dominance quickly allows axillary buds to grow (suckers) resulting in yield loss...
  9. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 26

    Identification of differentially expressed axillary bud specific genes and their promoters

    KUDITHIPUDI C.; SHEN Y.; XU D.; FREDERICK J.; YANG J.; WAREK U.; STRICKLAND J.
    Altria Client Services LLC, Research, Development and Regulatory Affairs, Richmond, VA, U.S.A.
    Tobacco plant exhibits exceptionally strong apical dominance. Molecular signals from shoot apical meristem mediate a hormonal environment that effectively inhibits axillary bud growth. Upon removal of the apical meristem, the signal is lost, enabling...