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12 results

  1. CORESTA Meeting, Smoke Science/Product Technology, 2021, Online, ST 38

    In vitro to in vivo extrapolation (IVIVE) for evaluating exposure and health impacts of e-vapor products using ingredient and mixture in vitro data

    ZHANG Jingjie(1); CHANG X.(2); HINES D.(2); BELL S.(2); LEE K.M.(1)
    (1) Altria Client Services LLC, Richmond, VA, U.S.A.; (2) Integrated Laboratory Systems (ILS), RTP, NC, U.S.A.
    In Vitro to In Vivo Extrapolation (IVIVE) uses reverse dosimetry to translate in vitro bioactivity data to clinically relevant exposure metrics. This study applies the IVIVE approach to e-vapor products using reported in vitro cytotoxicity data of...
  2. CORESTA Meeting, Smoke Science/Product Technology, 2021, Online, ST 50

    In vivo genotoxicity testing of aerosols generated from JUUL ENDS products

    LALONDE G.(1); ZHANG Jingjie(2); JALIGAMA S.(3); GUPTA A.(3); PSURNY E.(3); LEE K.M.(2); DOSHI U.(2); YAO J.(1)
    (1) JUUL Labs, Inc., San Francisco, CA, U.S.A.; (2) Altria Client Services, LLC, Richmond, VA, U.S.A. (a Service Provider to Juul Labs, Inc.); (3) Battelle Memorial Institute, West Jefferson, OH, U.S.A.
    We evaluated the in vivo genotoxicity potential of aerosols generated from two commercial nicotine electronic delivery systems (ENDS) products, P1 and P2, with a combined micronucleus (MN) and comet study design based on OECD TG 474 and...
  3. TSRC, Tob. Sci. Res. Conf., 2019, 73, abstr. 067

    Characterization of smokeless tobacco products extracted with different solvents for in vitro testing

    ZHANG JINGJIE (1); DOSHI U.(1); THAIKOOTTATHIL J.(1); LEE K.M.(1); WOLZ R.L.(2)
    (1) Altria Client Services, Richmond, VA, USA; (2) Enthalpy Analytical, Richmond, VA, USA
    Unlike cigarettes, there are no standardized methods available for preparing extracts from smokeless tobacco (SLT) products for in vitro toxicological evaluation. Methods are available for Harmful and Potentially Harmful constituents (HPHCs)...
  4. TSRC, Tob. Sci. Res. Conf., 2019, 73, abstr. 105

    Preclinical testing of flavors in e-vapor products - Part 3: In vitro cytotoxicity and genotoxicity of representative flavor mixtures

    DOSHI U.B.; ZHANG JINGJIE; KUMAR A.; LEE K.M.
    Altria Client Services, Richmond, VA, USA
    Flavor compounds “generally recognized as safe” for oral consumption are commonly used in inhalable e-vapor products for which insufficient safety data exists. The hazard characterization of each flavor and various flavor mixtures is resource and...
  5. TSRC, Tob. Sci. Res. Conf., 2019, 73, abstr. 106

    Preclinical testing of flavors in e-vapor products - Part 4: Flavor transfer from the liquid to the aerosol for inhalation exposure

    ZHANG Jingjie; SMITH C.; ANDERSON C.; McCUTCHEON N.; MILLER J.H.; LEE K.M.
    Altria Client Services, Richmond, VA, USA
    Many flavor compounds used in e-liquids are generally recognized as safe (GRAS) for oral consumption. However, the respiratory effects of flavors in e-vapor aerosols are unknown. Preclinical inhalation studies can provide toxicity data to assess the...
  6. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, ST 68

    E-cigarette aerosol dynamics in a physical model of the adult human oral/pharyngeal/tracheal cavity

    WANG Qing; CASTRO N.D.; ZHANG Jingjie; LI Weiling; PITHAWALLA Y.B.; OLDHAM M.J.; LIPOWICZ P.; ROSTAMI A.A.
    Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    The objective of this work is to generate experimental data to validate a computational deposition model for e cigarette aerosol. An adult human oral/pharyngeal/tracheal hollow prototype model was generated using a 3D printer from the CT scan of a 28...
  7. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 34

    Characterization of an air-liquid-interface (ALI) in vitro exposure system (VITROCELL® VC1/7 and Ames 48) using a prototype e-vapor product

    ZHANG Jingjie(1); DOSHI U.(1); OLDHAM M.J.(1); COFFA B.G.(1); GILLMAN I.G.(2); WOLZ R.L.(2); KOSACHEVSHY P.(2); LEE K.M.(1)
    (1) Altria Client Services LLC, Richmond, VA, U.S.A.; (2) Enthalpy Analytical, Inc., Richmond, VA, U.S.A.
    Direct delivery of aerosol or vapor to the apical surface of cells (ALI) allows more relevant exposure for in vitro toxicological evaluation of inhalable chemicals. In this study, we quantitatively characterized the aerosol delivery in a commercially...
  8. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 61

    A 6-month inhalation study in Apoe-/- mice to investigate cardiovascular and respiratory exposure effects of e-vapor aerosols compared with cigarette smoke

    WONG Ee Tsin(1); SZOSTAK J.(2); KRISHNAN S.(1); TAN Wei Teck(1); SEOW E.(1); TENG C.(1); LEE K.M.(3); ZHANG Jingjie(3); McKINNEY W.J.(3); PEITSCH M.C.(2); VANSCHEEUWIJCK P.(2); HOENG J.(2)
    (1) Philip Morris International Research Laboratories Pte. Ltd., (part of Philip Morris International group of companies), PMI R&D, Singapore; (2) Philip Morris Products S.A. (part of Philip Morris International group of companies), PMI R&D, Neuchâtel, Switzerland; (3) Altria Client Services LLC, Richmond, VA, U.S.A.
    Chronic exposure to cigarette smoke is a risk factor for the development and progression of cardiovascular disease and chronic obstructive pulmonary disease. Considerable attention has been given towards the potential reduced harm of e cigarettes (e...
  9. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 009 (also presented at CORESTA 2018)

    Toxicological assessment of e-liquid formulations using in vitro genotoxicity and cytotoxicity assays

    COFFA B.G.; DOSHI U.; ZHANG Jingjie; McKINNEY W.J.; LEE K.M.
    Altria Client Services LLC, Richmond, VA, USA
    In the Electronic Nicotine Delivery Systems (ENDS) Premarket Tobacco Application (PMTA; 2016) Draft Guidance, the FDA recommends a full assessment of the toxicological profile associated with new tobacco products, using in vitro toxicology (e.g.,...