Search Results

12 results

  1. CORESTA Meeting, Smoke Science/Product Technology, 2013, Seville, STPOST 14

    Validation of the VitroCell VC10 smoke exposure system for in vitro assays

    FIELDS W.R.(1); FOWLER K.W.(1); HARGREAVES V.H.(2); WALKER L.D.(2); BOMBICK B.R.(1)
    (1) R.J. Reynolds Tobacco Co., R&D, Winston-Salem, NC, U.S.A.; (2) Genetic & Molecular Toxicology, Covance Laboratories Ltd., Harrogate, U.K.
    The VITROCELL VC10® smoke exposure system offers multiple platforms and air liquid interface (ALI) exposure to mimic in vivo-like conditions for assessing the toxicological impact of smoke in in vitro assays. The validation plan to ensure the system...
  2. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SS 05

    Evaluation of a panel of potential gene biomarkers in two in vitro lung models following exposure to tobacco smoke and extracts

    FIELDS W.R.(1,2); LIEDTKE W.(2); LI J.(2); BOMBICK B.R.(1)
    (1) R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA; (2) Integrated Toxicology and Environmental Health Program, Duke University, Durham, NC 27710, USA
    Lung cells are frequently used in in vitro studies to assess the impact of cigarette smoke on defense and repair mechanisms of the lung. Previously, we identified a panel of fifteen genes representing inflammation, oxidative stress, metabolism and...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2011, Graz, STPOST 05

    Comparison of gene expression profiles of cigarette smoke-exposed normal human bronchial epithelial (NHBE) to profiles from smokers and patients with chronic obstructive pulmonary disease (COPD) and lung cancer

    FIELDS W.R.; BOMBICK B.R.
    R.J. Reynolds Tobacco Company, R&D, Winston-Salem, NC, USA
    Molecular changes in multiple signaling pathways that regulate oxidative stress, inflammation and xenobiotic metabolism are frequently observed in diseased lung. The purposes of this study were: 1) to assess differential gene expression profiles in...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2011, Graz, STPOST 06

    Evaluation of cigarette smoke-induced cell transformation using three in vitro cell transformation assays

    FOWLER K.; FIELDS W.R.; NORDSKOG B.K.; POTTS R.; BOMBICK B.R.; PANT K.
    (1) R.J. Reynolds Tobacco Co., R&D, Winston-Salem, NC, USA; (2) BioReliance Corporation, Rockville, MD, USA
    Cell transformation assays (CTA) detect phenotypic changes associated with neoplastic transformation and available evidence suggests in vitro cell transformation may stimulate early components of the multistage process of carcinogenesis. Moreover,...
  5. CORESTA Congress, Edinburgh, 2010, SSPTPOST 22; TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 07

    Gene expression analyses of the liver From ApoE-/- mice exposed to mainstream cigarette smoke

    NORDSKOG B.K.; CURTIN G.M.; BROWN J.E.; BOMBICK B.R.
    R.J. Reynolds Tobacco Company, R&D, Winston-Salem, NC, USA
    The goal of this study was to identify key molecular alterations induced by mainstream smoke exposure and diet in a genetically susceptible mouse model of atherosclerosis. ApoE-deficient female mice were exposed nose-only to mainstream cigarette...
  6. CORESTA Congress, Edinburgh, 2010, SSPTPOST 24; TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 10

    Standardization of the preparation of smokeless tobacco extracts for assessment of biological effects

    PRASAD G.L.; FOWLER K.; HILL J.A.; BOMBICK B.R.
    R.J. Reynolds Tobacco Company, R&D, Bowman Gray Technical Center, Winston-Salem, NC, USA
    Exposure to smokeless tobacco (ST) has been reported to elicit diverse biological effects at the cellular level. Currently, there is no consensus regarding parameters that will impact cellular response. Various conditions have been described for 1)...