Search Results

299 results

  1. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length sequence ...
  2. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SS 05

    Evaluation of a panel of potential gene biomarkers in two in vitro lung models following exposure to tobacco smoke and extracts

    FIELDS W.R.(1,2); LIEDTKE W.(2); LI J.(2); BOMBICK B.R.(1)
    (1) R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA; (2) Integrated Toxicology and Environmental Health Program, Duke University, Durham, NC 27710, USA
    Lung cells are frequently used in in vitro studies to assess the impact of cigarette smoke on defense and repair mechanisms of the lung. Previously, we identified a panel of fifteen genes representing inflammation, oxidative stress, metabolism and ...
  3. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPT 22

    Development of biomarkers of effect from chronic tobacco usage. Part 1: Study design and biomarkers of exposure

    JONES B.A.; CHEN P.; SCHMIDT E.; PRASAD G.L.
    R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA
    To assess the effects of chronic exposure to combustible and non-combustible tobacco product use, a single site, cross-sectional clinical study was conducted. Three cohorts of healthy males (40/cohort, 35-60 years) were enrolled: long-term smokers and ...
  4. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPT 23

    Development of biomarkers of effect from chronic tobacco usage. Part 2: Inflammation and oxidative stress

    PRASAD G.L.; JONES B.A.; CHEN P.; SCHMIDT E.
    R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA
    In a recent clinical study, we investigated the long-term effects of smoking and moist snuff consumption using a panel of biomarkers of effect (BioEff) indicative of inflammation, oxidative stress and lipid metabolism. In this, cross-sectional study, we ...
  5. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPT 24

    Development of biomarkers of effect from chronic tobacco usage. Part 3: Potential metabolomics biomarkers of tobacco effect

    PRASAD G.L.(1); CHEN P.(1); JONES B.A.(1); KENNEDY A.D.(2)
    (1) R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA; (2) Metabolon, Durham, NC, USA
    Epidemiological data indicate that consumption of moist snuff is associated with reduced harm relative to cigarette smoking. However, a need exists for interim biomarkers of effect (BioEff) that would be useful to assess the health effects of tobacco. To ...
  6. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPTPOST 06

    Combusted, but not smokeless tobacco product preparations, cause DNA damage in human oral cavity cells

    ZACHARIAS W.(1); GAO H.(1); PRASAD G.L.(2)
    (1) Dept. of Medicine, J.G. Brown Cancer Center, University of Louisville, Louisville, KY, USA; (2) R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA
    We examined the effects of reference tobacco preparations on DNA damage in human oral cavity cells. The oral squamous cell carcinoma cell line (101A), normal human gingival epithelial cells (HGEC), and human gingival fibroblasts (HGF) were treated with ...
  7. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPTPOST 36

    Development of biomarkers of effect from chronic tobacco usage.Part 4: Metabolomic profiles from cigarette smokers and moist snuff consumers

    PRASAD G.L.(1); CHEN P.(1); JONES B.A.(1); KENNEDY A.D.(2)
    (1) R.J. Reynolds Tobacco Co., R&D Department, Winston-Salem, NC, USA; (2) Metabolon, Durham, NC, USA
    The long-term health effects associated with cigarette smoking have been shown to be more harmful compared to those associated with the consumption of non-combustible tobacco products, such as moist snuff. In order to investigate the long-term effects of ...
  8. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 07

    Complete artificial saliva alters expression of proinflamma-tory cytokines in human dermal fibroblasts

    MALPASS G.E.(1); ARIMILLI S.(1); PRASAD G.L.(2); HOWLETT A.C.(1)
    (1) Wake Forest University Health Sciences, Winston-Salem, NC, USA; (2) R.J. Reynolds Tobacco Company, Winston-Salem, NC, USA
    Complete artificial saliva (CAS) is a saliva substitute often used as a vehicle for test articles, including, smokeless tobacco. We discovered, using polymerase chain reaction gene expression array analyses, that CAS increased gene expression for certain ...