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50 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product....
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according...
  5. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 54

    The regulation of autophagy of susceptible Nicotiana tabacum under tobacco mosaic virus infection

    YANG Jinguang(1); LIU Xaingfu(1); LIU Wei(1); QIAN Yumei(1); ZHANG Jing(2); LI Wei(2); DONG Shifei(2); WANG Yaofeng(3); HUANG Mingdi(4); WANG Fenglong(1)
    (1) Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Qingdao, P.R. China; (2) Hongyun Honghe Tobacco (Group) Co., Ltd., Kunming, P.R. China; (3) Qingyang Branch of Gansu Province Tobacco Company, Xifeng, P.R. China; (4) Longnan Tobacco Company, Gansu Tobacco Cooperation, Wudu 746000, P.R. China
    Autophagy (or self-eating) is an important conserved process by which eukaryotic cells recycle intracellular components. This study was undertaken to elucidate whether tobacco mosaic virus (TMV) infection on tobacco plants induced autophagy. TMV-U1...
  6. CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, ST 27

    Analysis of process intensity on tobacco moisture retention based on uniform design

    LI Wenjuan(1); ZHOU Fangfang(1); LONG Minghai(1); ZI Wenhua(1); JIANG Meihong(1); LIU Ze(2)
    (1) Yunnan Reascend Tobacco Technology (Group) Co., Ltd., Kunming, Yunnan, P.R. China; (2) Hongyun Honghe Group, Kunming, Kunming, P.R. China
    Tobacco moisture retention provides essential information about moisture adsorption behaviour and physical characteristics of cigarette formulating, which also influence cigarette aroma and smoking quality. Effect of the intensity of the casing...