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65 results

  1. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 11

    Field trials with low nicotine tobacco varieties developed by conventional breeding technique: first results

    MALPICA A.(1); EVANS W.(2); ARCHIBALD C.(3); SCOTT L.(4); GARWE D.(5); CHAMANGO A.M.Z.(6); DORLHAC de BORNE F.(7); COLARD S.(8)
    (1) Bergerac Seed and Breeding, Bergerac, France; (2) Imperial Tobacco South Africa Pvt. Ltd, Umhlanga, Durban, South Africa; (3) Alliance One International Services, Camberley, Surrey, U.K.; (4) Universal Leaf Tobacco Co., Richmond, VA, U.S.A.; (5) Tobacco Research Board (TRB), Harare, Zimbabwe; (6) Agricultural Research & Extension Trust (ARET), Lilongwe, Malawi; (7) Imperial Tobacco Limited, Bergerac, France; (8) SEITA - Imperial Brands plc, Paris, France
    In 2015, the World Health Organisation (WHO) Study Group on Tobacco Product Regulation (“TobReg”) issued an advisory note recommending a strategy of reducing nicotine in tobacco to substantially lower levels of 0.4 mg/g. In the U.S., the Food and...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As...
  3. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product....
  4. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 099a & 99b

    Machine made cigar leaf and product workshop

    STEVENS R.(1); BLACK R.(1); GILLMAN I.G.(2); SMITH J.(3); HARTLEY M.(4); LINDEGAARD T.(5)
    (1) ITG Brands LLC, Greensboro, NC, USA; (2) Enthalpy Labs, Durham, NC, USA; (3) Altria Client Services LLC, Richmond, VA, USA; (4) Universal Dark Air-Cured Group, Oxford, NC, USA
    The regulation of tobacco products continues to be a growing focus around the world. For example, in 2016, the FDA finalized a rule in the USA that extended its regulatory authority to all tobacco products, including e-cigarettes, cigars, hookah, and...
  5. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c...