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89 results

  1. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  2. Tobacco Science & Technology, 2012, 8, p. 54-59, 65, ISSN.1002-0861

    Correlation analysis between nicotine content and medium and trace element contents in flue-cured tobacco leaves from different stalk positions

    CHEN Wei(1); TANG Yuanju(1); PAN Wenjie(1); LI Zhiyong(*2)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) Guizhou Provincial Tobacco Company, Guiyang 550003, P.R. China
    The relationships between nicotine content and the contents of medium and trace elements in flue-cured tobacco leaves from different stalk positions were analyzed with descriptive statistics analysis, stepwise regression analysis and path analysis. The ...
  3. Tobacco Science & Technology, 2012, 8, p. 70-74, ISSN.1002-0861

    Cloning and sequence analysis of β-carotene hydroxylase gene in Nicotiana tabacum

    LIN Shifeng; WANG Rengang; Wang Yi; REN Xueliang*
    Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China
    In order to effectively modulate the anabolism of carotenoid in tobacco, a β-carotene hydroxylase (BCH) gene related to plant carotenoid anabolism was cloned from Nicotiana tabacum. A cDNA sequence of encoded BCH gene was isolated from tobacco cv. K326 by ...
  4. Tobacco Science & Technology, 2012, 8, p. 79-83, ISSN.1002-0861

    Preliminary analysis of soil microbial diversity in tobacco bacterial wilt disease nursery

    CAI Liuti(1,2); SHANG Shenghua(1); HU Zhongyi(1); WANG Lili(2); LIU Yanxia(2); WANG Hancheng(1); YANG Xingming(2); SHI Junxiong(*1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Resource and Environmental Science, Nanjing Agricultural University, Nanjing 210095, P.R. China
    To prevent and control tobacco bacterial wilt disease with antagonistic bacterium against Ralstonia solanacearum, the microbial diversity in soil of tobacco bacterial wilt disease nursery was preliminarily analyzed by means of conservative fragment ...
  5. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 12

    Bacteriophage can reduce Ralstonia solanacearum induced stem necrosis in tobacco

    FORTNUM B.A.; PETERSON P.D.
    Clemson University, Dept of Entomology Soils and Plant Sciences, Pee Dee REC, Florence, SC, USA
    Bacterial wilt caused by Ralstonia solanacearum is an extremely damaging disease of flue-cured tobacco in the south-eastern USA and can be spread mechanically on farm machinery. Rolastonia solanacearum exhibits strong tissue-specific tropism within the ...
  6. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 16

    The effect of growth regulators on the mechanical transmission of bacterial wilt

    PETERSON P.D.; FORTNUM B.A.
    Clemson University, Dept of Entomology Soils and Plant Sciences, Pee Dee REC, Florence, SC, USA
    Mechanical transmission of Ralstonia solanacearum during flower and leaf removal has contributed significantly to the spread and severity of bacterial wilt of tobacco in the south-eastern USA. Mechanical topping and harvesting also coincide with the time ...
  7. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 18

    The study and development of protection equipment for pesticide application and harvesting tobacco

    CASTANHEIRA L.C.
    Independent Consultant - Safety and Hygiene in Rural Labor, Campinas - SP, Brazil, SindiTabaco – Interestate Tobacco Industry Union, Brazil
    Tobacco growers are exposed to pesticides during crop protection applications and also to alkaloids during harvest periods. Throughout the year 2009 a study was carried out to evaluate and analyse all aspects regarding the legislation and technical ...
  8. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 05

    Cloning and gene structure analysis of T-psy1 from flue-cured tobacco

    CAI Liuti(1); HU Zhongyi(1); LEI Lu(1); LI Jixin(2)
    (1) Guizhou Tobacco Research Institute, Guizhou Provincial Tobacco Company, Guiyang, P.R China; (2) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Carotenoid is an important precursor of tobacco aroma, and phytoene synthase is one of the key enzymes in the pathway of aroma precursors biosynthesis and accumulation in tobacco. Based on the homology cloning method, the cDNA and DNA sequence of the ...
  9. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 07

    Comparative transcriptome of tobacco leaves in typical ecological areas of Guizhou

    LEI Bo(1); PAN Wenjie(1); DING Fuzhang(1); ZHANG Kai(2); LI Jinxin(3); LU Ku(2)
    (1) Guizhou Tobacco Research Institute, Guiyang, P.R China; (2) College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R China; (3) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Flue-cured tobacco (Nicotiana tabacum) is a sensitive crop to ecological environment, and distinctive tobacco has always formed under special ecological conditions. In order to investigate the molecular mechanism of formation of medium and light aroma ...