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12 results

  1. CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, AP 41

    Effects of different growth temperatures on alkaloid content and nicotine metabolism in roots, stems and leaves of tobacco (Nicotiana tabacum L.) plants

    JIN Y.F.(1); WANG S.S.(1); LI J.Y.(2); PANG T.(2); ZHANG J.B.(1); GONG M.(1)
    (1) School of Life Sciences, Yunnan Normal University, Kunming, P.R. China; (2) Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China
    Alkaloids, especially nicotine content, significantly influences quality and flavour types of tobacco leaves, which is differentially regulated by various environmental conditions. To investigate the effects of different growth temperature on...
  2. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length...
  3. Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

    Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

    WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
    (1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China
    In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain...
  4. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers...
  5. Tobacco Science & Technology, 2012, 4, p. 72-75, ISSN.1002-0861

    Structural characteristic analysis and mRNA expression of Cathepsin B gene in Nicotiana tabacum

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2,3)
    (1) Technology Center of China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China; (3) Institute of Agronomy and Life Science, Chongqing University, Chongqing 400030, P.R. China
    In order to clarify the function of Cathepsin B gene (NbCathB) in Nicotiana tabacum, the sequence of NbCathB was analyzed. The NbCathB ORF (Open Read Frame) was 951 bp in length and encoded 316 amino acids with the predicted molecular weight of 35.2...
  6. Tobacco Science & Technology, 2012, 7, p. 72-75, ISSN.1002-0861

    Analysis of differential protein in tobacco leaves sprayed with microbial agent MP

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2)
    (1) Technology Center, China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China
    In order to find out the effects of Microbial agent MP (Bacillus magaterium) spraying on the quality of tobacco leaves, two-dimensional gel electrophoresis (2-DE) was used to analyze the differences of protein expression between tobacco leaves...
  7. Tobacco Science & Technology, 2012, 9, p. 58-62, ISSN.1002-0861

    Selective removal of hydrogen cyanide from cigarette smoke by combined filter of coptis alkaloid

    WU Jinfeng(1,2); LI Xuegang(1); WANG Peng(3); PENG Zhong(3); LAN Zhongyu(3); ZHENG Lifeng(2)
    (1) College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P.R. China; (2) Chongqing Cigarette Factory, Chongqing Tobacco Industrial Co., Ltd., China Tobacco Chuanyu Industrial Co., Ltd., Chongqing 400061, P.R. China; (3) Department of Technology, Chongqing Tobacco Industrial Co., Ltd., China Tobacco Chuanyu Industrial Co., Ltd., Chongqing 400060, P.R. China
    Coptis alkaloids were extracted from rhizome of Coptis chinensis rich in quaternary ammonium hydroxide and added into cigarette filter. On the basis of the reduction of hydrogen cyanide delivery in cigarette smoke, the way of coptis alkaloid adding...
  8. Tobacco Science & Technology, 2012, 9, p. 75-81, ISSN.1002-0861

    Cloning and sequence analysis of carotenoid cleavage dioxygenase gene from Nicotiana tabacum

    GAO Junping(1); WANG Genhong(1,2); CHENG Tingcai(1,2); DUAN Jun(1,2); XIA Qingyou(*2)
    (1) Institute of Agriculture and Life Science, Chongqing University, Chongqing 400044, P.R. China; (2) State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, P.R. China
    In order to reveal the function of carotenoid cleavage dioxygenases (CCDs) in the growth and development of tobacco plants, a CCD4 (NtCCD4) gene was cloned from Samsun oriental tobacco by RT-PCR. According to sequence analysis, with a coding sequence...
  9. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 07

    Comparative transcriptome of tobacco leaves in typical ecological areas of Guizhou

    LEI Bo(1); PAN Wenjie(1); DING Fuzhang(1); ZHANG Kai(2); LI Jinxin(3); LU Ku(2)
    (1) Guizhou Tobacco Research Institute, Guiyang, P.R China; (2) College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R China; (3) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Flue-cured tobacco (Nicotiana tabacum) is a sensitive crop to ecological environment, and distinctive tobacco has always formed under special ecological conditions. In order to investigate the molecular mechanism of formation of medium and light...
  10. Tobacco Science & Technology, 2010, 6, p. 77-81., ISSN.1002-0861

    Influences of carbendazim on AM fungus infected tobacco seedlings and mycorrhizal effect

    XIE LI(1,2); DUAN YU-QI(2); JIN YAN(2); YANG YU-HONG(2); CAI MIN(1); HUANG JIAN-GUO(1)
    1. College of Resources and Environmental Sciences, Southwest University, Chongqing 400716, P.R. China; 2. Yunnan Academy of Tobacco Agricultural Sciences, Yuxi 653100, P.R. China
    In order to investigate the influences of carbendazim on AM fungus infected tobacco seedlings and mycorrhizal effect, the experiment of AM fungus inoculating and carbendazim applying was conducted in a seedling floating system. The results showed...