Search Results

1837 results

  1. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 04

    Quantitative analysis for metabolic profiling of aroma compounds by near infrared spectroscopy (NIR)

    YANG Panpan(1,2); ZHOU Wenzhong(1,2); YANG Xiaoyun(1,2); LI Ming(1); LU Xiaoting(1,2); LIU Jing(1,2)
    (1) Yunnan Reascend Tobacco Technology (Group) Co., Ltd, Kunming, China; (2) Yunnan Comtestor Co., Ltd, Kunming, China
    It is well-known that the types and content of aroma components in tobacco is an important focus of the cigarette industry. Different types and contents of aroma components are the reaction of different metabolic profiling. This study proposed...
  2. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 05

    Identification of a novel mode of action of vanillin derivative compound veratraldehyde

    WATANABE T.; MUNAKATA S.; TAKAHASHI T.; HASHIZUME T.
    Japan Tobacco Inc., Scientific Product Assessment Center, Yokohama, Kanagawa, Japan
    Compounds that test positive in in vitro micronucleus tests (MNTs) need to be followed up with in vivo MNTs. However, it is common for these compounds to test negative in in vivo MNTs, known as “misleading positives.” To reduce in vivo follow-up...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 06

    Validation of extraction method for tobacco-free nicotine pouches and Swedish snus pouch products - towards standardization of in vitro testing

    TALIKKA M.; GORALCZYK A.; DOS SANTOS D.; ALRIQUET M.; ZANETTI F.; ORTEGA TORRES L.; PAK C.; BERTHOUZOZ M.
    PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland
    Tobacco-free nicotine pouches (NP) and tobacco-containing Swedish snus pouches (Swedish snus) are two types of nicotine-delivery products for oral use. A standard method to generate an extract of these products for in vitro testing has not been...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 07

    In vitro cytotoxicity and mechanistic insight from MucilAir™ and SmallAir™ tissues exposed to cigarette smoke and next-generation products

    HOLLINGS M.(1); ROTHWELL E.(1); MARTIN S.(1); DAUNT A.(1); FREIBERG G.(2); BEDFORD R.(1)
    (1) Labcorp Early Development Laboratories Ltd., Harrogate, U.K.; (2) Labcorp Early Development Laboratories Ltd., Eye, U.K.
    There is a current push to investigate emerging Next Generation Products (NGPs) including Electronic Nicotine Delivery System (ENDS) and Heated Tobacco Products (HTPs). 3D airway tissue models are a relevant method for analysis of toxicity for these...
  5. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 08

    Aerosol generation and exposure atmosphere characterization of VITROCELL Air Liquid Interface (ALI) exposure system using ENDS products

    GUPTA A.; PSURNY E.; NEWSWANGER J.; GRIFFIN C.; AMELUNG A.; BEHRINGER S.; MOYER B.
    Battelle Memorial Institute, Columbus, OH, U.S.A.
    Electronic nicotine delivery systems (ENDS) are frequently tested to evaluate their potential as reduced-risk alternatives to combustible cigarettes. This involves a variety of tests including in vivo and in vitro inhalation toxicity evaluations. A...
  6. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 09

    Examination of automated growth inhibition classification in Ames test by machine learning

    KUM R.; KAIYA K.; ITO H.
    Japan Tobacco Inc., Scientific Product Assessment Center, Yokohama, Kanagawa, Japan
    The Ames test is a commonly used bacterial bioassay to assess the mutagenicity of chemicals and has been applied to evaluate the mutagenicity of tobacco products. In Ames test, a statistically significant dose-related increase in revertant colonies...
  7. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 10

    In vitro micronucleus in V79 cells using Microflow - treatment at the air liquid interface with aerosol from three different nicotine containing products

    ROTHWELL E.; SMITH G.; MARTIN S.; HOLLINGS M.
    Labcorp Early Development Laboratories Ltd, Harrogate, U.K.
    The in vitro micronucleus assay is a standard genetic toxicology test. Historically this has been run with a slide scoring endpoint which is labour intensive and low throughput for testing multiple products. Flow cytometer analysis can be performed,...
  8. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 11

    Validation of the Vitrocell® HTP 2.0+ 12 well mammalian module for assessing cigarettes, ENDS, and eHTPs: evaluation of dilution airflow, dose resolution, and dose repeatability

    SEYMOUR A.; ROTHWELL E.; MARTIN S.; HOLLINGS M.
    Labcorp Early Development Laboratories Ltd, Harrogate, U.K.
    The present study aimed to validate the performance of the Vitrocell® HTP 2.0+ 12 well mammalian module for assessing three different test articles: cigarettes, electronic nicotine delivery systems (ENDS), and electronic tobacco heated products...
  9. CORESTA Meeting, Smoke Science/Product Technology, 2023, Cancun, STPOST 12 (also presented at TSRC 2023)

    Rapid in vitro toxicological screening using ToxTracker to determine the effect of repeated freeze/thaw of combustible cigarette extract

    CARMINES E.(2); MISRA M.(2); OH S.(1); HENDRIK G.(3); COFFA B.G.(1,2); ALEKSA K.(1)
    (1) Labstat International, Kitchener, ON, Canada; (2) Chemular Inc., Hudson MI, U.S.A.; (3) Toxys Inc., CG Leiden, The Netherlands
    Combustible cigarette extracts (CCE) are stored at -80 °C to preserve sample integrity. Sample shipping and repeated freeze/thaw cycles prior to testing may lead to changes in the chemical composition through the formation of ice-water...