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4318 results

  1. Tobacco Science & Technology, 2012, 3, p. 34-37., ISSN.1002-0861

    Cloning and sequence analysis of T-zds1 gene from flue-cured tobacco

    HU Zhongyi(1) ; LEI Bo(1) ; LIU Dong(2) ; CAI Liuti(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agriculture, Yangtze University, Jingzhou 434023, Hubei, P.R. China
    Homology cloning and in silico cloning method were used to clone the full length cDNA sequence of tobacco zeta carotene dehydrogenase gene (named T-zds1) from flue-cured tobacco cultivar, Jiucaiping2, and the primary and secondary structures of its...
  2. Tobacco Science & Technology, 2012, 3, p. 38-47., ISSN.1002-0861

    Effects of ambient temperature and relative humidity on migration behavior of tobacco flavors in cigarettes

    BAI Xinliang(1) ; HU Jun(1), WANG Jun(2); ZHOU Jianhua(2); CHU Guohai(2); HU Youchi(1); ZENG Shitong(1); ZHAO Mingyue(1); YANG Jun(2)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310009, P.R. China
    In order to investigate the effects of ambient temperature and relative humidity (RH) on the migration behavior of tobacco flavors in cigarette, the migration ratios of flavors to mainstream smoke (MS) and their retention ratios by filter tips...
  3. Tobacco Science & Technology, 2012, 3, p. 48-51., ISSN.1002-0861

    Factors influencing determination of total volatile components in tobacco flavors

    LIU Shan(1); HU Jun(1); LIU Yang(1); HUANG Yan(2); ZHU Qinglin(3); QU Rongfen(4); LIU Yi(5); YANG Tingshu(6); XIE Jianping(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Huafang Tobacco Flavor Co., Ltd., Guangzhou 510530, P.R. China; (3) China Tobacco Anhui Industrial Co., Ltd., Hefei 230088, P.R. China; (4) Hongta Tobacco (Group) Co., Ltd., Yuxi 653100, Yunnan, P.R. China; (5) Huabao Food Flavor and Fragrance (Shanghai) Co., Ltd., Shanghai 201821, P.R. China; (6) Yunnan Reascend Tobacco Technology (Group) Co., Ltd., Kunming 650106, P.R. China
    In order to investigate the factors influencing the determination of total volatile components in tobacco flavor, 6 tobacco flavor samples including top flavors and casings were measured in 4 laboratories. The factors involved were specifications of...
  4. Tobacco Science & Technology, 2012, 3, p. 52-56., ISSN.1002-0861

    Determination of phthalic acid esters in cigarette filter by GC/MS

    ZHANG Hongfei; LIU Nan; BIAN Zhaoyang; TANG Gangling; HU Qingyuan
    China National Tobacco Quality Supervision & Test Center, Zhengzhou 450001, P.R. China
    A method for simultaneously determining 17 phthalic acid esters (PAEs) in cigarette filter was established with GC/MS. The parameters such as extraction mode, extraction solvent, extraction condition, detection condition were tested, and 45 cigarette...
  5. Tobacco Science & Technology, 2012, 3, p. 60-63., ISSN.1002-0861

    Correlations of ammonia in mainstream cigarette smoke with ammonia, nitrite and nitrate in tobacco leaf

    YU Hongxiao; ZHAO Yantang; XU Haitao; ZHAO Yueli; MA Qiang
    Technology Center of China Tobacco Shandong Industrial Co., Ltd., Jinan 250100, P.R. China
    In order to investigate the correlations between the delivery of ammonia in mainstream cigarette smoke and the contents of nitrite, nitrate and ammonia in tobacco leaf, the delivery of ammonia in mainstream smoke and the contents of nitrite, nitrate...
  6. Tobacco Science & Technology, 2012, 3, p. 64-66, ISSN.1002-0861

    Determination of chlorine in tobacco soil with continuous flow analysis

    ZHANG Haiyan; TAO Xiaoqiu; HUANG Mei
    Sichuan Tobacco Quality Supervision & Test Station, Chengdu 610041, P.R. China
    Aiming at the wide pH range and low chlorine content in Sichuan tobacco soil, a continuous flow analysis method for determining chlorine in tobacco soil was developed. The chlorine in tobacco soil was extracted with water and separated by centrifugal...
  7. Tobacco Science & Technology, 2012, 3, p. 67-74, ISSN.1002-0861

    Variance analysis of carotenoid and its degradation product contents in flue-cured tobacco of different appearance

    XI Yuanxiao(1,2); LIU Yang(1); LI Feng(1); YANG Jun(1); WANG Xinmin(1); WEI Chunyang(1); SONG Jizhen(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Shandong Weifang Tobacco Company, Weifang 261061, Shandong, P.R. China
    The contents of carotenoid and its degradation products were determined by HPLC and GC/MS, respectively. The results showed that: 1) The contents of lutein, β-carotene and carotenoid increased with the darkening of leaf color, decreased with the...
  8. Tobacco Science & Technology, 2012, 3, p. 75-78, ISSN.1002-0861

    Effects of barn types on quality of Yunyan85 cured leaves

    LI Ping; GUO Qunzhao; PENG Guoxun
    Liangshan State Company of Sichuan Provincial Tobacco Corporation, Xichang 615000, Sichuan, P.R. China
    For studying the effects of barn types on cured tobacco leaf quality, the traditional barn (c1), upgraded conventional curing barns (c2) and down draught bulk curing barn (c3) were compared. The results showed that the barns in the order of the...
  9. Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

    Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

    WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
    (1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China
    In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain...