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  1. Tobacco Science & Technology, 2012, 4, p. 37-43, ISSN.1002-0861

    Determination of 11 polycyclic aromatic hydrocarbon biomarkers in human urine with LC-MS/MS

    CHEN Yusong; WANG Sheng; YU Jingjing; LIU Huimin*; CAO Depo
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    In order to evaluate the exposure to PAHs in environmental tobacco smoke, the influences of solid phase extraction column, chromatographic column and mass spectrometry conditions on analytical results were investigated, a method with SPE-HPLC/MS/MS...
  2. Tobacco Science & Technology, 2012, 4, p. 44-47, ISSN.1002-0861

    Determination of arsenic and lead in polypropylene tow binder by direct injection GF-AAS

    GE Shaolin(1); ZHU Dongliang(1); HU Lizhong(1); NIU Jiajia(2); HAN Yunhui(2)
    (1) Technology Center, China Tobacco Anhui Industrial Co., Ltd., Hefei 230088, P.R. China; (2) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    A direct injection-graphite furnace atomic absorption spectrometry (GF-AAS) method for determining arsenic (As) and lead (Pb) in polypropylene tow binder was developed. The results showed that, twentyfold dilution and additional drying process at 120...
  3. Tobacco Science & Technology, 2012, 4, p. 48-50, 66, ISSN.1002-0861

    Study on residue and degradation of deltamethrin in Oriental tobacco

    YANG Shaosong(1); CHEN Jing(*1); TANG Xubing(2); LUO Lin(2); YAO Min(2)
    (1) Institute of Agricultural Environment and Resource, YAAS, Kunming 650205, P.R. China; (2) Baoshan Oriental Tobacco Company, Baoshan 678000, Yunnan, P.R. China
    In order to assess the degradation behavior of deltamethrin residues in oriental tobacco, the field experiments were conducted for two consecutive years with 2.5 % deltamethrin EC. The test results indicated that: 1) The degradation of deltamethrin...
  4. Tobacco Science & Technology, 2012, 4, p. 5-8, ISSN.1002-0861

    Characterization of cut tobacco size distribution based on fractal theory

    YU Na(1); SHEN Xiaofeng(2); XU Dayong(1); LI Huajie(3); CHANG Mingbin(3); DU Jinsong(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Technology Center of Hongyun Honghe Tobacco (Group) Co., Ltd., Kunming 650202, P.R. China; (3) Technology Center of China Tobacco Fujian Industrial Co., Ltd., Xiamen 361004, Fujian, P.R. China
    In order to study the characteristics of cut tobacco size distribution, the correlation equation of accumulative mass percentage of cut tobacco under test sieve versus fractal dimension was derived, and the relationship between loss of tobacco from...
  5. Tobacco Science & Technology, 2012, 4, p. 51-55, ISSN.1002-0861

    Comparison on cytotoxicity of cigarette smoke components trapped at each stage in cambridge filter-solvent-solvent systems

    YAO Jianhua; MI Qili; GENG Yongqin; YANG Yekun; HUANG Haitao; LI Xuemei; MIAO Mingming; TANG Ping
    Yunnan Academy of Tobacco Science, Kunming 650106, P.R. China
    In order to investigate the effects of cigarette smoke trapping on in vitro cytotoxicity test, 3 kinds of trapping systems, including Cambridge filter-pH7.0 PBS-acetone, Cambridge filter-pH7.2 PBS-ethyl acetate and Cambridge filter-pH7.4 PBS-70 %...
  6. Tobacco Science & Technology, 2012, 4, p. 56-59, 66, ISSN.1002-0861

    Analysis of residues of famoxadone and fenamidone in tobacco

    ZHU Baokun(1); LIAO Yuqi(2); JIANG Juxing(1); WANG Sujuan(1); LIU Ya(1); XIA Jianjun(*1)
    (1) Hongyun-Honghe Tobacco (Group) Co., Ltd., Kunming 650202, P.R. China; (2) School of Chemical Science and Technology, Yunnan University, Kunming 650091, P.R. China
    A method for fast testing the famoxadone and fenamidone residues in tobacco leaves with ultra-high performance liquid chromatography was developed by using acetonitrile as an extraction solvent. Twenty-two flue-cured tobacco samples were tested. The...
  7. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers...
  8. Tobacco Science & Technology, 2012, 4, p. 67-71, ISSN.1002-0861

    Quantification analysis of leaf color of flue-cured tobacco from shandong and its relationship with polyphenol and carotenoid contents

    LIANG Taibo(1); ZHANG Yanling(1); YIN Qisheng(*1); WEI Chunyang(1); CAI Xianjie(1); WANG Jianwei(1); TIAN Lei(2); YU Wenming(2)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Linyi Branch of Shandong Provincial Tobacco Company, Linyi 276003, Shandong, P.R. China
    In order to investigate the relationship between color characteristics and chromogenic substances of the flue-cured tobacco leaves from Shandong, the cured leaf was sampled and leaf color was quantification analyzed, and the relationships between...
  9. Tobacco Science & Technology, 2012, 4, p. 72-75, ISSN.1002-0861

    Structural characteristic analysis and mRNA expression of Cathepsin B gene in Nicotiana tabacum

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2,3)
    (1) Technology Center of China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China; (3) Institute of Agronomy and Life Science, Chongqing University, Chongqing 400030, P.R. China
    In order to clarify the function of Cathepsin B gene (NbCathB) in Nicotiana tabacum, the sequence of NbCathB was analyzed. The NbCathB ORF (Open Read Frame) was 951 bp in length and encoded 316 amino acids with the predicted molecular weight of 35.2...
  10. Tobacco Science & Technology, 2012, 4, p. 76-80, ISSN.1002-0861

    Inductive effects of elicitors on enzyme activity and aroma component accumulation in tobacco leaves

    ZANG Xiaojing(1); LI Yongkuan(2); ZHAO Gaoling(1); DU Lei(1); GAO Ming(3); XI Yu(1); ZHU Daheng(*1)
    (1) Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, P.R. China; (2) Zhengzhou Cigarette Factory, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450004, P.R. China; (3) Yellow River Basin Water Resources Protection Bureau, Zhengzhou 450004, P.R. China
    The influences of elicitors on the activity of key secondary metabolism enzymes and accumulation of aroma components in tobacco leaves were studied with pot experiments by spraying elicitors on leaf surface. The results showed that the enzyme...