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  1. Tobacco Science & Technology, 2011, 2, p. 47-51., ISSN.1002-0861

    Determination of seven preservatives in tobacco flavor by RP-HPLC

    TIAN HAI-YING(1); SONG JIN-YONG(1); WANG HONG-WEI(1); WANG HONG-XIA(1); XING LIANG(1); WANG SHENG(2)
    1. Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450000, P.R. China; 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    A method for simultaneously determining 7 preservatives in tobacco flavors by reversed-phase high performance liquid chromatography (RP-HPLC) with a variable wavelength detector (VWD) was developed. The parameters such as extraction solvent,...
  2. Tobacco Science & Technology, 2011, 2, p. 52-57., ISSN.1002-0861

    Application of euclidean-mahalanobis distance in pattern recognition of flavor fingerprint chromatograph

    ZHAO YUE-LI(1); YU HONG-XIAO(2); ZONG ZHAO-QI(1); MA YING(3); Lü CHENG-JUN(1)
    1. Tengzhou Station, Technology Center of China Tobacco Shandong Industrial Corporation, Tengzhou 277500, Shandong, P.R. China; 2. Qingdao Station, Technology Center of China Tobacco Shandong Industrial Corporation, Qingdao 266101, Shandong, P.R. China; 3. Qingzhou Station, Technology Center of China Tobacco Shandong Industrial Corporation, Qingzhou 262500, Shandong, P.R. China
    A method for evaluating the fingerprint chromatographs of a mixed system by Euclidean-Mahalanobis distance was put forward. The Euclidean-Mahalanobis distances of GC/MS fingerprint chromatographs of six standard samples and two mixture samples, and...
  3. Tobacco Science & Technology, 2011, 2, p. 58-63., ISSN.1002-0861

    Cloning and sequence analysis of T-psy1 gene from flue-cured tobacco

    CAI LIU-TI(1); JIANG GUANG-HUA(2); LEI BO(1); HU ZHONG-YI(1)
    1 .Guizhou Tobacco Research Institute, Guiyang 550018, P.R. China; 2. Guizhou Provincial Tobacco Company, Guiyang 550003, P.R. China
    Adopting a homology cloning method, the full length cDNA sequence of tobacco phytoene synthase gene (named T-psy1) was cloned from flue-cured tobacco cv. K326, the T-psy1 gene GenBank accession number HM345582, included 1521bp, with coding protein...
  4. Tobacco Science & Technology, 2011, 2, p. 70-74., ISSN.1002-0861

    Comparison of carotenoid and polyphenol contents and smoking quality between different flue-cured tobacco cultivars

    XI YUAN-XIAO; SONG JI-ZHEN; YANG JUN; LIU YANG; GUO WEI-MIN
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    The contents of carotenoid and polyphenol in leaves of five flue-cured tobacco cultivars grown in Yunnan in 2009 were quantitatively determined with reverse phase high performance liquid chromatography, and the smoking quality of the tobacco was...
  5. Tobacco Science & Technology, 2011, 2, p. 9-12., ISSN.1002-0861

    Factors affecting ash integration of burning cigarette

    CHENG ZHAN-GANG; YE MING-QIAO; HU SU-XIA; WANG JIAN-XIN; CHEN HUI-BIN
    Technology Center, China Tobacco Hubei Industrial Co., Ltd., Wuhan 430051, P.R. China
    In order to accurately evaluate the property of ash integration of burning cigarette, a 20-channel linear smoking machine was designed and the images of ash left on burning cigarettes were taken by a digital camera. The results showed that: 1) when...
  6. Tobacco Science & Technology, 2011, 7, p. 28-32., ISSN.1002-0861

    Simultaneous determination of residues of 28 organophosphorus pesticides in tobacco by gas chromatography with dual columns and double detectors

    LI QIAN-KUN(1); YANG YI-NAN(2)
    1. Guangxi Subtropical Crops Research Institute, Nanning 530001, P.R. China; 2. China Tobacco Guangxi Industrial Co., Ltd., Nanning 530001, P.R. China
    For rapidly detecting the residues of 28 organophosphorus pesticides in tobacco, a gas chromatography (GC) with double columns and dual detectors was developed via investigating the effects of sample pretreatment conditions and chromatographic...
  7. Tobacco Science & Technology, 2011, 7, p. 33-36., ISSN.1002-0861

    Extraction of tobacco materials for paper-making process reconstituted tobacco in presence of enzyme mixture

    WU YI-JI; SHEN GUANG-LIN; TAO HONG; ZHOU RONG; LIN XIANG
    Technology Center of China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510145, P.R. China
    In order to reduce the undesirable components in paper-making process reconstituted tobacco, the effects of adding pectinase, hemicellulase and protease in tobacco fines and stems extraction, adding rates, enzymatic hydrolysis temperature and time on...
  8. Tobacco Science & Technology, 2011, 7, p. 43-47., ISSN.1002-0861

    Determination of fifteen pesticide residues in tobacco with LC-MS/MS

    YAN HUI-HUI(1); HU BIN(2); LIU HUI-MIN(2); MA HAI-MING(1); WANG LI-JIE (1); SHI JIE(1)
    1. Department of Chemistry, Zhengzhou University, Zhengzhou 450001, P.R. China; 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    A method for rapidly determining fifteen pesticides, such as pymetrozine and others, in tobacco with LC-MS/MS was developed. Samples were extracted by acetonitrile, cleaned up by dispersive solid-phase extraction and finally detected with LC-MS/MS....
  9. Tobacco Science & Technology, 2011, 7, p. 5-9, 27., ISSN.1002-0861

    Analysis of chemical components affecting cigarette smoke mildness and development of mildness enhancing flavor

    YANG LEI; YANG QING; FENG HONG-TAO; XU GUANG-JIN
    Technology Center of Hongyun Honghe Tobacco Group Co., Ltd., Kunming 650202, P.R. China
    In order to enhance the mildness of cigarette smoke and improve smoking quality, the chemical components in tobacco and cigarette smoke were determined with GC-MS, and their correlations with mildness of cigarette smoke were analyzed. The results...
  10. Tobacco Science & Technology, 2011, 7, p. 52-55., ISSN.1002-0861

    Determination of calcium and magnesium in cigarette paper with autoanalyzer

    WANG PENG; SHANG JUN; LÜ XIANG-MIN; ZHU LI-JUN; LI LI
    China Tobacco Chuanyu Industrial Corporation, Chengdu 610066, P.R. China
    The effects of digestion solution volume and digestion temperature on the results of calcium and magnesium contents in cigarette paper determined by an autoanalyzer were investigated, and 20 cigarette paper samples were detected paralleled with ionic...