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  1. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 16

    Application of protoplast technology facilitates the CRISPR-Cas9 mediated gene replacement in Nicotiana tabacum and confers resistance against tobacco mosaic virus

    YUAN Cheng; HUANG Changjun; LIU Yong; ZENG Jianmin; TONG Xhijun; YU Haiqin; FANG Dunhuang; XIAO Bingguang
    Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan Province, China
    Tobacco mosaic virus (TMV) is an important virus pathogen that threatens the production of tobacco. In the past 50 years, only the N gene has been deployed for TMV-resistant tobacco breeding, while the TMV-resistant flue-cured tobacco varieties...
  3. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 18

    Use of exotic Nicotiana tabacum germplasm for confronting an inverse genetic correlation in flue-cured tobacco

    LEWIS R.S.
    Department of Crop and Soil Science, North Carolina State University, NC, U.S.A.
    Inverse genetic correlations between cured alkaloid accumulation and cured leaf yields in flue-cured tobacco complicate the development of higher-yielding cultivars while maintaining alkaloids at commercially acceptable levels. Introgression of...
  4. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 19

    Effects of drought stress on changes in morphology and expression of selected genes in tobacco

    PRZYBYŚ M.
    Institute of Soil Science and Plant Cultivation – State Research Institute, Pulawy, Poland
    Global climate change observed in recent decades indicates an alarming trend of increasing extreme weather events, including the emergence of more frequent and longer periods of drought. The increasing extent of drought in areas devoted to tobacco...
  5. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 20

    Integrative analysis of transcriptome and metabolome provides insights into the underlying mechanism of cold stress response and recovery in two tobacco cultivars

    HU Zhengrong(1); YAN Weijie(2);YANG Chenkai(2); LI Yangyang(1); YANG Jiameng(1); HU Risheng(1)
    (1) Hunan Tobacco Research Institute, Changsha, Hunan, China; (2) College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China
    Low temperature is one of major environmental factors limiting the growth, quality and yield of tobacco. However, the molecular mechanism of tobacco cold response remains largely unknown. Here, integrated biochemical, transcriptomic and metabolomic...
  6. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 21

    Loss of susceptibility loci in tobacco for the development of durable resistance to black shank

    FREDERICK J.; PRAMOD S.; ADAMS A.; XU D.; LUSSO M.
    Altria Client Services LLC, Richmond, VA, U.S.A.
    Black shank root and crown rot disease caused by the soil-borne pathogen, Phytophthora nicotianae, is detrimental to commercial tobacco (Nicotiana tabacum) production and is responsible for significant economic loss. Numerous naturally occurring...
  7. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 22

    Distribution of the MLO gene mutations for powdery mildew resistance in tobacco cultivars

    IWAI Y.; ARAI M.; KOMATSU T.; UDAGAWA H.; TAJIMA T.; SATO S.
    Japan Tobacco Inc., Leaf Tobacco Research Center, Oyama, Tochigi, Japan
    Powdery mildew is a fungal disease affecting various crops, causing serious yield losses under environments with high humidity and moderate temperature. The Japanese domestic tobacco (Nicotiana tabacum L.) cultivar ‘Kokubu’ shows high powdery mildew...
  8. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 23

    Functional characterization of transcription factor NtERF13a in regulating phenylpropanoids biosynthesis in tobacco

    WANG Zhong(1); ZHANG Zhan(2); LUO Zhaopeng(1); LIU Pingping(1); WU Mingzhu(1); LI Zefeng(1); ZHANG Jianfeng(1); XU Xin(1); YANG Yongfeng(2); YANG Jun(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan Province, China; (2) Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan Province, China
    Tobacco leaves contain abundant phenylpropanoids compounds, including chlorogenic acid, rutin, scopoletin, and lignin. These phenylpropanoids are important for plant growth and development, and participate in plant resistance to various environmental...
  9. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 24

    Development of a mutant library and a high-quality reference genome for forward genetics in tobacco wild relatives Nicotiana sylvestris

    TAKEUCHI T.; ARAI M.; UDAGAWA H.; MAGOME H.; TAJIMA T.; TAKAKURA Y.
    Japan Tobacco Inc., Leaf Tobacco Research Center, Oyama, Tochigi, Japan
    Tobacco (Nicotiana tabacum) is an allotetraploid species with complex genome structure. This complexity prevents tobacco from forward phenotypic screen of gene-knockout mutations because their effect can be masked by the corresponding homeologous...
  10. CORESTA Congress, Online, 2022, Agronomy/Phytopathology Groups, AP 25

    Single-cell transcriptome of Nicotiana tabacum leaves reveals developmental trajectories of glandular trichome

    CHEN Hongyu; LI Xiaohan; FAN Longjiang
    Institute of Crop Science and Institute of Bioinformatics, Zhejiang University, Hangzhou, China
    Leaf glandular trichomes of Nicotiana tabacum represent a biologically active and stress-responsive tissue that contributes to plant defence response against biotic and abiotic stress and also influences leaf aroma and smoke flavour. Although a...