The regional spatial variation and distribution of available microelements in tobacco growing soil at a county level were studied with GIS and geostatistics by taking Changtu County in Liaoning Province as an example. The results showed that the spatially variant functions for available Fe, Mn, Cu, Zn, B and Mo contents in soil could be expressed by Gaussian, Gaussian, spherical, exponential, exponential and exponential models, respectively. Among them, available Cu, Zn and B contents assumed moderate spatial correlations and were affected by structural factors (such as parent material, topography and soil type) and random factors (such as fertilization and field managements); while available Fe, Mn and Mo contents showed weak spatial correlations and were mostly affected by random factors. Kriging interpolation map indicated that: the distribution of available Fe, Mn, Cu, Zn, B and Mo contents showed a trend of high-low from south to north in general. Relatively, the distribution patterns of Cu, Zn and B were more complicate, their contents increased gradually from southwest to northeast in northern region, decreased from the north of middle area to surroundings in southern region.