Potted cultivation was used to study the chloride distribution in different tobacco plant organ, and the relation of chloride content with soil, applied fertilizer and irrigated water in tobacco leaf. The results showed that the dried weight would decrease with the applied chloride amount increased. The highest chloride content organ of tobacco plant was leaf, followed by stem. The root was the lowest. The distribution of chloride in the different leaf position was that the lugs was highest, followed by the cutters, and the tops was the lowest. In the tobacco plant, 55.1%~67.6% chloride was located in the leaf, 19.8~26.5% of which was in stem, and 12.6~18.4% of which in root system. The 61.8% chloride in tobacco plant was from soil, 37.1% from irrigated water and only 1.1% from applied fertilizer. It was proved that Soil and irrigated water were the main chloride origins in tobacco plant. Excess chloride resulted in poisoning, the dry matter decrease, nitrogen and potassium absorption restraint, and obvious potassium content decrease in tobacco leaf.