Three genes, including K+ transporter AtKup1, Na+/H+ anti-porter AtNHX1 and inorganic pyrophosphatase AVP2 were cloned from Arabidopsis and transformed into tobacco. The transcription patterns of five tobacco internal genes were evaluated using qRT-PCR methods. The genes encoding K+ channel, K+ transporter, vacuole H+-PPase, plasma membrane H+-ATPase and vacuole H+-ATPase were analyzed in transgenic and wild type tobacco plants under potassium starvation, and sodium and ammonia stress conditions. The results demonstrated that the transcript of the NtHAK1 gene was reduced under all treatments, and NHA1 was increased in the roots of AtKup1 transformants. The NtHAK1 gene transcript was also downregulated in the roots of AtNHX1 transformants, but the NHA1 and VAG1 transcripts encoding H+-ATPase were significantly upregulated. The NVP1 gene, encoding vacuolar H+-PPase increased slightly. VAG1 encoding H+-ATPase and NVP1 encoding vacuolar H+-PPase transcripts were downregulated. The NtHA-K1 and NKT1 transcripts were slightly increased in the AVP2 gene overexpressed in tobacco roots. In addition, transcripts of five tobacco internal genes were analyzed under different nutrition and salt stress conditions. The study demonstrated that the transcription of NtHAK1 and NHA1 were significantly stimulated, while the expression of NVP1 dccreased in response to an external K+ starvation solution treatment. The results also confirmed that the K+ transporter gene NtHAK1 transcript was induced by excessive Na+ stress, but the transcription of the gene was inhibited when the tobacco plants were in a 5 mmol/L NH4+ solution. NKT1 transcript levels exhibited no response to potassium starvation and sodium or ammonium stress treatments, suggesting that NKT1 is constitutively expressed.