Some compounds in tobacco leaf are transferred to cigarette paper by moisture. The purpose of this study was the identification and quantitative analysis of the high polarity transferred compounds on to cigarette paper. The transferred materials were extracted with distilled water using ultrasonic extraction at 50 °C. The extract was purified with column chromatography, filled with C18. The purified fractions were collected and re-purified in the same manner. The purified fraction was analyzed by gas chromatography / mass spectrometry (GC/MS). Four polyphenol compounds, benzathiazole, Salicyl alcohol, Formyl nornicotine, 4-Cumyl phenol, and Scopoletin peaks were observed in the GC/MS profile. Among them, scopoletin had very strong fluorescence intensity with very low concentration at ex 342 nm / em 460 nm. This property of scopoletin enabled a quantitative analysis of transferred materials on the cigarette paper. Scopoletin was quantified by HPLC equipped with a fluorescence detector, and fluorescence profile was measured by fluorescence spectrophotometer. From this study, we found that the moisture in cigarette was directly proportional to transferred material amount on cigarette paper.