CORESTA Meeting, Agronomy/Phytopathology, Montreux,1997, AP57

Transgenic tobacco for efficient control of Orobanche spp

VALKOV V.; ATANASSOVA S.; SLAVOV S.; BOSSOLOVA S.; BATCHVAROVA R.; ATANASSOV A.
Institute of Genetic Engineering, Kostinbrod, Bulgaria.
Several structurally distinct classes of herbicides including the sulfonylurea (Glean), imidazolinone (Pivot) as well as asulam (Asulox, Rhône-Poulenc) and Basta (Hoechst AG) were used for transformation of tobacco cultivars via Agrobacterium tumefaciens . Four different constructs, carrying genes for resistance to these herbicides were used. The obtained transformants were grown in greenhouse conditions and tested for different herbicide resistance. Some of the herbicide resistant plants were transferred into an artificial system for studying the effect of herbicides against broomrape ( Orobanche spp.) infestation at different parasitic stages (germination, attachment and development). The system gives opportunities to study the early stages of host-parasite interactions as well as the effect of the applied herbicides for Orobanche spp control. PCR analyses and DNA hybridisation were performed to prove the integration of the foreign genes. Transformation with well-characterised mutant genes into transgenic plants have a great potential in manipulating the spectrum of herbicides species and also to extend the range of their applications for possibilities to control Orobanche spp. infestation in tobacco fields.