Pythium seedling blight has been observed in greenhouse float systems in the southeastern USA resulting in irregular seedling growth with necrotic roots. Replacement of transplants prior to setting can reduce net farm income by 20% if seedlings are purchased late in the transplant season. The usability of Pythium infected transplants was evaluated in field trials where P. myriotylum infected plants were transplanted to the field and evaluated for yield and quality. The experimental design was a 2 factor factorial with four replications and the test was repeated. Seedlings were grown in simulated float bays where water temperatures were held constant at 15, 20, 25 and 30°C. Seedlings were inoculated with P. myriotylum and the bays incubated for 21 days prior to transplanting. Foliage of Pythium infected seedlings prior to transplanting was greener in color than non-infected seedlings. Infestation of greenhouse float water with P. myriotylum did not alter cured leaf yield, leaf quality, value and root necrosis in field tobacco grown from these seedlings (Trial 1 and 2, P≤0.05). Roots were evaluated for nematode ( Meloidogyne incognita race 3) induced galling. Root galling was lower in plants grown in float water that was infested with P. myriotylum when compared to plants grown in non-infested water (Trial 2) (P ≤ 0.025).