No. 12 - Determination of Alkaloids in Cigarette Smoke Condensate
(CRM developed into ISO 3400)
An aliquot of a methanol solution of a cigarette smoke condensate is steam distilled in two steps: the neutral and acid steam-volatile substances are removed by distillation after acidification of the solution with mineral acid and the pyridine alkaloids (nicotine) are subsequently distilled from the same solution made strongly alkaline. The absorbance of the distillate from the alkaline distillation is measured by ultraviolet spectrophotometry and the alkaloid content is calculated as nicotine. This method is applicable to methanol solutions of cigarette smoke condensates.