This method is applicable for determination of seven aromatic amines in mainstream cigarette smoke by Gas Chromatography / Mass Spectrometry in Negative Chemical Ionization mode (GC/MS-NCI). The method was also shown to be appropriate for analysis of aromatic amines in mainstream cigarette smoke (both ISO and intense smoking regimes) up to 14 mg/cigarette ISO Nicotine Free Dry Particulate Matter (NFDPM).