Reports current

Joint Experiment Technical Study (JETS) Report 16/2 Maleic Hydrazide in Tobacco

AA Technical Report

March 2017 Ref. AA-111-CTR

The ISO 4876:1980 method (Tobacco and tobacco products - Determination of maleic hydrazide residues), was systematically reviewed in 2011. Two revision proposals were submitted to the ISO/TC 126 from that time, suggesting changing the principle of the official method to be adopted. Whilst the current version of the ISO method is based on caustic extraction and colorimetric measurement, both submitted proposals are based on the acidic extraction condition.

During the annual meeting of the CORESTA Sub-Group Agrochemical Analysis (SG AA) in 2012, an internal trial showed different capabilities of the tested methods to extract residues of naturally incurred Maleic Hydrazide (MH). Similar methods that were submitted to the ISO committee were tested in the mentioned internal trial.

Due to the complexity of the distribution of MH throughout the tobacco plant, the SG AA decided to conduct a Joint Experiment Technical Study (JETS) to assess both MH fate and how to analyze it correctly.  This JETS report describes a proficiency test to evaluate the capacity of different methods - especially those ones submitted to ISO as proposal to replace the current official method - to properly measure MH content in both artificially spiked samples and naturally incurred tobacco.