This Recommended Method specifies a method for the determination of the content of ‘Total Sugars’ as glucose in tobacco by CFA using hydrochloric acid (HCl) for hydrolysis and p-hydroxybenzoic acid hydrazide (PAHBAH) for colour formation. This method is applicable to unprocessed tobacco lamina and processed tobacco such as cigarette blend tobacco and roll-your-own (RYO) tobacco.