Tobacco Science & Technology, 2007, 12, p. 55-59, ISSN.1002-0861.
Construction of PVY and CMV binary virus resistant RNA silencing vector
Shenyang Agricultural University, Shenyang, China
Using RT-PCR method, the conserved fragment of PVY CP gene, PVY HC-Pro gene, CMV CP gene and CMV 2b gene were cloned, then the fragments of PVY CP and CMV 2b, PVY HC-Pro and CMV 2b, PVY HC-Pro and CMV CP were connected respectively. Three RNA...