Tob. Sci., 1973, 17-47, p. 145-148, ISSN.0082-4523
An efficient method for producing and studying tobacco brown-spot disease in the laboratory
Oxford Tobacco Research Laboratory, Southern Region, Agricultural Research Service, United States Department of Agriculture, Oxford, North Carolina USA; North Carolina State University, Raleigh, North Carolina USA
A method is described for efficiently producing brown-spot disease in the laboratory, using leaf discs cut from tobacco plants grown in the greenhouse. Discs were inoculated by placing drops of water containing conidia of the fungal pathogen,...