Tob. Sci., 1984, 28-13, p. 41-45, ISSN. 0082-4523
Preparation and some chemical and physical properties of proteases in tobacco leaves
Department of Plant Pathology, University of Kentucky, KY, USA; U.S D.A., Agricultural Research Service, Lexington, KY, USA
Serine protease, carboxypeptidase and sulfhydryl protease in tobacco leaf extract were precipitated out with 30-60% ammonium sulfate saturation. In comparison with fresh tissues, storage at -70°C did not cause protease activity to decrease; whereas...