Tobacco Science & Technology, 2007, 4, p. 40-44, ISSN.1002-0861
Isolation, purification and trait of Cu, Zn SODs in tobacco leaves
Technology Center of Nanjing Cigarette Factory, Nanjing, China
Three isoenzymes, Cu, Zn-superoxide dismutases I, II and III, were isolated from ripe fresh tobacco leaves (K326, Nicotiana ) by extraction with 50 mmol/L, pH7.6 phosphate buffer solution (PBS), successive precipitation with 55%-60% (w/w) ammonium...