CORESTA Meeting, Smoke/Technology, Xian, 2001, ST 20
The purification and properties of polyphenol oxidase from tobacco
Chongqing Tobacco Industrial Co. Ltd, Chongqing, China, Qingliang Liu University of Science and Technology of China.
Polyphenol oxidase (PPO 1.14.18.1) was prepared by acetone extraction of powdered fresh tobacco leaves and purified by ammonium sulfate precipitation and column chromatography with DEAE-Sephadex A-50, CM-Sephadex C-50 and Sephadex G-75. Two proteins...