2016 Collaborative Study on Minor Alkaloids in Tobacco Products
RAC-STS Technical Report
From 2013 to 2016, the CORESTA Routine Analytical Sub-Group (RAC) and Smokeless Tobacco Sub-Group (STS) conducted a series of collaborative studies for the determination of nornicotine and anabasine in tobacco and tobacco products. A gas chromatography mass spectrometry (GC-MS) method using methanol as the extract solvent and 6-methylquinoline and d4-nornicotine as the internal standards was eventually selected.
This document reports on the collaborative study undertaken in 2016 to calculate repeatability and reproducibility (r & R) values and determine if the methodology was suitable for the purpose of determining nornicotine and anabasine in tobacco and tobacco products.