A novel LC-MS technique has been developed for the analysis of several organic acids in tobacco and smokeless tobacco products. The analyzed acids include oxalic, quinic, pyruvic, malic, lactic, citric, fumaric and maleic. Acetic acid cannot be measured by the new method. The separation of the acids has been performed on a Synergy 4u Hydro-RP column 250 x 4.6 mm. The mobile phase consisted of a aqueous solution containing 5% methanol and 0.15% formic acid. The flow rate in the column was 0.6 mL/min and the injection was of 5 ul. The detection was performed on a LC-MS/MS API 5000 system from AB-Sciex working in negative mode. Although multiple reaction monitoring (MRM) type acquisition was used, the product and precursor ions were the same with declustering potential at -10V, collision energy at -5V, and collision gas flow rate of 3 mL/min. These mild conditions assured the high sensitivity of the method. The ions monitored for analysis had m/z = 87 for pyruvic acid, 89 for oxalic and lactic acids, 115.1 for maleic and fumaric acids, 133.1 for malic acid, and 191.1 for quinic and citric acids. Deuterated lactic acid and citric acid were used as internal standards. The technique was validated using typical validation criteria for an analytical method. Using this new technique, several samples of tobacco leaf, snus, and wet snuff were analyzed.