A new method was needed to analyze low-level TSNA concentrations in electronic cigarette liquid. This proved difficult with the various flavored matrices, however the method was developed to accurately quantitate TSNAs as low as 50 parts per trillion for N-nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosoanatabine (NAT) ; concentrations of N-nitrosoanabasine (NAB) are accurately quantitated at 13 parts per trillion. An Agilent 1290 Infinity II LC paired with an AB Sciex 6500+ Mass Spectrometer were used for analysis. Chromatographic separation was achieved using a Phenomenex® Gemini® 3µm C18, 110Å 150 x 2 mm column. This method has been validated and the results will be presented.