Soxhlet extraction with n-hexane of milled tobacco mixed with Florisil is applicable only for the determination of very apolar organochlorine pesticides (CORESTA Recommended Method N° 2). In the case of the determination of more polar pesticides (e.g. organophosphorus pesticides) polar extraction solvents are necessary to obtain reasonable recoveries. But due to possible coextracted interferences from the tobacco matrix it is mandatory to add a more selective clean-up step prior to GC analysis or to identify compounds of interest by the application of a selective detection system (e.g. atomic emission detection, mass selective detection) after their GC separation. It is obvious that the combination of both is the most powerful tool. Data will be presented on the combination of different clean-up steps with different GC detection systems. Sample clean-up is performed using a automated sample preparation module ("PrepStation"). This module provides unattended automation of many sample functions and therefore eases the workload of technicians by performing repetitive tasks. The benefits and limitations of this system and the results obtained will be discussed.