Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
(1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China

In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and JN375578). The results of bioinformatics analysis showed that the full-length of genomic DNA and cDNA were 5027 bp and 1188 bp, encoding 317 amino acid residues. NtOR possessed 2 transmembrane domains, 1 signal peptide cleavage site, 5 glycosylation sites and 11 phosphorylation sites, and the secondary structure was mainly composed of 12 α-helices and 20 β-sheets, subcellular located on plasma membrane. The phylogenetic and BLAST analyses indicated that NtOR was the orthologous gene of SlOR and ViOR. Microarray results in GENEVESTIGATOR database revealed that NtOR was expressed at the highest level in cotyledon, followed by mature leaf, young shoot and flower, rather weak in other tissues and organs.