The contents of hemicellulose, cellulose, acid detergent lignin and coarse cellulose in 31 tobacco samples were determined by Fibertec2010 using quartz sand as filter aid. The results showed that: 1) The relative standard deviations (RSDs) of hemicellulose, cellulose, acid detergent lignin and coarse cellulose contents in tobacco, which were simultaneously detected by 6 analytical columns of Fibertec2010, were 2.82%, 1.71%, 4.79%, and 1.77%, respectively, and those of 4 repetitive determinations by one column were 1.61%, 1.06%, 3.72%, and 1.80%. 2) For the samples of same cultivar, there were no obvious differences of hemicellulose, cellulose, acid detergent lignin and coarse cellulose contents between different stalk positions. 3) The contents of cellulose and coarse cellulose in Burley tobacco and reconstituted tobacco were significantly higher than that in flue-cured tobacco. 4) The hemicellulose content in high grade cigarettes was higher than that in low grade cigarettes, while the contents of cellulose, acid detergent lignin and coarse cellulose were on the contrary.