The conductivity of impregnant and activity of relative enzymes of primed and unprimed naked tobacco seed were determined, and the characteristics of adult seedlings and root development of primed and unprimed pelleted seed were investigated. The results showed that: 1) for the primed naked seed, the conductivity of impregnant was reduced significantly, and the vigor of seed and activity of enzymes were increased comparing with untreated seed; 2) comparing with unprimed pelleted seed, the root activity, dry matter weight, weight ratio of dry matter in root to that in stalk and leaves, number of side roots, contents of chlorophyll, and activity of peroxidase (POD) and nitrate reductase (NR) of primed pelleted seed increased obviously. Therefore, primed pelleted tobacco seed feature higher seed vigor, well-developed root and better seedling property.